玉米螟虫生真菌球孢白僵菌几丁酶基因的克隆与分析

球孢白僵菌是最常见的昆虫病原真菌之一,已成功应用于玉米螟和松毛虫等害虫的生物防治。球孢白僵菌主要通过分泌多种胞外水解酶降解昆虫体壁侵入寄主,其分泌的水解酶主要包括蛋白酶、几丁质酶、脂酶等。本研究从玉米螟虫生真菌球孢白僵菌中分离到一个几丁质酶基因BbChitI,该基因的编码区包含1047bp,编码了348个氨基酸,氨基酸序列与来源于小菜蛾虫生真菌的白僵菌几丁质酶的同源性高达99%,与来源于木霉菌的几丁质酶的同源性达到82%。

Cloning and Analysis of a Chitinase Gene BbChitI from Beauveria bassiana Isolated from Ostrinia Furnacalis

Beauveria bassiana is one of the entomopathogenic fungi and has been successfully applied to bio-control Ostrinia furnacalis and Dendralimus punctatus. Beauveria bassiana mainly depends on cuticle degrading enzymes including proteases, chitinases and lipases to penetrate host's cuticle. In the present study, a chitinase gene BbChitI was firstly cloned from Beauveria bassiana isolated from Ostrinia furnacalis. Sequence analysis revealed a cDNA sequence of 1047bp long and encoded an open-reading from consisting of 348 amino acids. The deduced amino acid sequence exhibited 99% and 82% identity to that of Cordyceps bassiana chitinase and Trichoderma harzianum chitinase respectively.