| 唐松草的组织培养与快速繁殖技术 |
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| 引用本文： | 王连翠,魏东. 唐松草的组织培养与快速繁殖技术[J]. 安徽农业科学, 2006, 34(10): 2098-2099 |
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| 作者姓名： | 王连翠 魏东 |
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| 作者单位： | 临沂师范学院生命科学学院,山东,临沂,276005;临沂师范学院生命科学学院,山东,临沂,276005 |
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| 摘 要： | 通过对唐松草的组织培养,研究了不同激素配比对愈伤组织、芽的诱导分化和增殖的影响以及驯化移栽时基质对于成活率的影响。结果表明:诱导愈伤组织的适宜培养基为MS+1.0 mg/L6-BA+0.5 mg/L2,4-D;诱导芽分化的适宜培养基为MS+2.0 mg/L6-BA+0.2 mg/LNAA;适宜继代培养基为MS+1.0 mg/L6-BA+0.2 mg/LNAA;适宜生根培养基为1/2 MS+0.5 mg/LNAA。同时还表明,炼苗时选用珍珠岩与蛭石以11∶的比例混合的基质的成活率最高。
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| 关 键 词： | 唐松草 组织培养 快速繁殖 |
| 文章编号： | 0517-6611（2006）10-2098-02 |
| 收稿时间： | 2006-03-01 |
| 修稿时间： | 2006-03-01 |
Tissue Cuture and Rapid Propagation of Thalictrum aquilegifolia |
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| WANG Lian-cui et al. Tissue Cuture and Rapid Propagation of Thalictrum aquilegifolia[J]. Journal of Anhui Agricultural Sciences, 2006, 34(10): 2098-2099 |
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| Authors: | WANG Lian-cui et al |
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| Affiliation: | Life Science Department, Linyi Normal University, Linyi, Shandong 276005 |
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| Abstract: | In this article,the influence of several factors on micropropagation of Thalictrum aquilegifolia was investigated.The results showed that the appropriate media for the callus induction was MS + 1.0 mg/L 6-BA + 0.5 mg/L 2,4-D,buds differentiation and subculture medium was MS + 2.0 mg/L 6-BA + 0.2 mg/L NAA,MS + 1.0 mg/L 6-BA + 0.2mg/L NAA,respectively;the best medium for initiation and growth was 1/2 MS + 0.5 mg/L NAA.The plantlets were transplanted to perlite and vermiculite(1 ∶1) where the plant grew well,and the survival rate was 85 %. |
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| Keywords: | Thalictrum aquilegifolia Tissue Cuture Rapid Propagation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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