| 苜蓿雄性不育系组织培养技术的研究 |
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| 引用本文： | 高翠萍,石凤翎,孙静. 苜蓿雄性不育系组织培养技术的研究[J]. 中国草地学报, 2006, 28(5): 29-32 |
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| 作者姓名： | 高翠萍 石凤翎 孙静 |
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| 作者单位： | 内蒙古农业大学生态环境学院,内蒙古,呼和浩特,010019 |
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| 摘 要： | 以苜蓿雄性不育系Ms-4幼嫩茎段、叶片、叶柄为外植体,诱导苜蓿雄性不育系再生植株.结果表明:叶柄是诱导愈伤组织的良好外植体,体细胞胚诱导率为90%,再生率49%.苜蓿雄性不育系插条生根的最佳处理为接种前用水进行预处理0.5h,外植体为完整插条,培养基为A1、A2,其生根率为97%,生根系数为5.3.
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| 关 键 词： | 苜蓿 雄性不育系 组织培养 体细胞胚 |
| 文章编号： | 1673-5021(2006)05-0029-04 |
| 修稿时间： | 2006-03-15 |
Studies on Tissue Culture Techniques of Male Sterile Line of Alfalfa |
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| GAO Cui-ping,SHI Feng-ling,SUN Jing. Studies on Tissue Culture Techniques of Male Sterile Line of Alfalfa[J]. Chinese Journal of Grassland, 2006, 28(5): 29-32 |
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| Authors: | GAO Cui-ping SHI Feng-ling SUN Jing |
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| Abstract: | The infancy stem segments,leaves and leafstalks of male sterile line Ms-4 of Medicago varia Martin.cv.Caoyuan No.1 were taken as explants and induced somatic embryos and roots of male sterile line Ms-4 of Medicago varia Martin.cv Caoyuan No.1.The results showed that leafstalks are good explants for inducing callus.Somatic embryos inducing rates was 90%,regeration rate was 49%.The best treatment of rooting of quickset of male sterile line Ms-4 of Medicago varia Martin.cv.Caoyuan No.1 is water treat for 0.5h before inoculabitity,integrity quickset,and A1 and A2 rooting medium,rooting rates was 97%,rooting coefficient was 5.3. |
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| Keywords: | Alfalfa Male sterile line Tissue culture Somatic embryos |
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