Real-time PCR for differential determination of the tomato wilt fungus, <Emphasis Type="Italic">Fusarium oxysporum</Emphasis> f. sp. <Emphasis Type="Italic">lycopersici</Emphasis>, and its races |
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| Authors: | Keigo Inami Chizu Yoshioka Yasushi Hirano Masato Kawabe Seiya Tsushima Tohru Teraoka Tsutomu Arie |
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| Institution: | (1) Laboratory of Plant Pathology, Tokyo University of Agriculture and Technology (TUAT), Fuchu Tokyo, 183-8509, Japan;(2) Saitama Prefectural Agriculture and Forestry Research Center, Kuki, Saitama, Japan;(3) National Agricultural Research Center (NARC), Tsukuba, Ibaraki, Japan;(4) National Institute for Agro-Environmental Sciences (NIAS), Tsukuba, Ibaraki, Japan; |
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| Abstract: | Five primer/probe sets to identify the tomato wilt pathogen, Fusarium oxysporum f. sp. lycopersici (FOL), and its three races selectively were designed based on the rDNA-intergenic spacer and avirulence genes. Real-time PCR using
genomic DNA from mycelia and soil DNA with the primer/probe sets allowed the successful identification of FOL and its races. |
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