| 羊痘病毒PCR检测方法的建立和试剂盒的研制 |
| |
| 引用本文： | 张志成,陈丽华,陆静静,张大淦,戴薇,陈钟鸣.羊痘病毒PCR检测方法的建立和试剂盒的研制[J].中国动物检疫,2011,28(2):44-46. |
| |
| 作者姓名： | 张志成 陈丽华 陆静静 张大淦 戴薇 陈钟鸣 |
| |
| 作者单位： | 金陵科技学院动物科学与技术学院,江苏南京,210038 |
| |
| 基金项目： | 南京市教育科学研究"十一五"规划课题，金陵科技学院科研基金 |
| |
| 摘 要： | 根据GenBank上已发表的羊痘病毒(CPV)的全基因序列,设计并合成了一对能特异性扩增羊痘病毒的引物,扩增产物大小约为445bp。通过对PCR方法的优化,建立了检测羊痘病毒的PCR方法,并研制出检测试剂盒。同时对试剂盒的敏感性,特异性和稳定性进行了研究。实验结果表明,该试剂盒具有良好的敏感性和特异性,稳定性在-20℃至少可以保存一年。
|
| 关 键 词： | 羊痘病毒 PCR检测试剂盒 敏感性 特异性 |
Construction of PCR for Detection of Capripox Virus and its Kit |
| |
| Zhang Zhicheng,Chcn Lihu,Lu Jingjing,Zhang Dagan,Dai Wei,Chen Zhongming.Construction of PCR for Detection of Capripox Virus and its Kit[J].China Journal Of Animal Quarantine,2011,28(2):44-46. |
| |
| Authors: | Zhang Zhicheng Chcn Lihu Lu Jingjing Zhang Dagan Dai Wei Chen Zhongming |
| |
| Institution: | (Department of Animal Science and Technology,Jinling Technology Institute; Nanjing 210038,China) |
| |
| Abstract: | On the basis of published genomic sequence of capripox virus in the GenBank,a pair of primers for specific amplification of capripox virus fragment were designed and PCR assay for detection of the virus was constructed. PCR amplified product was 445bp. And,the test kit to detect capripox virus was developed. The experiments about kit’s sensitivity,specificity and expiration date were conducted. The result suggested the kit had high sensitivity and specificity. It could be stored at least for one year at-20℃. |
| |
| Keywords: | CPV PCR Kit Sensitivity Specificity |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国动物检疫》浏览原始摘要信息 |
| 点击此处可从《中国动物检疫》下载免费的PDF全文 |
|
