| 西瓜EST-SSR分子标记的筛选 |
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| 引用本文： | 邹明学,陈艳红,徐勇,郭绍贵,张海英,宫国义.西瓜EST-SSR分子标记的筛选[J].长江蔬菜,2009(18):13-14. |
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| 作者姓名： | 邹明学 陈艳红 徐勇 郭绍贵 张海英 宫国义 |
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| 作者单位： | 1. 南通大学,江苏南通,226007
2. 国家蔬菜工程技术研究中心 |
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| 摘 要： | 利用国际共享获得的820条EST序列,自主设计出79对EST-SSR引物,其中有32对引物经过PCR扩增出稳定清晰的电泳条带,可以作为西瓜和相关物种的新的EST-SSR分子标记。
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| 关 键 词： | 西瓜 EST-SSR 分子标记 |
Selection of Watermelon EST-SSR Molecular Marker |
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| ZOU Mingxue,CHEN Yanhong,XU Yong,GUO Shaogui,ZHANG Haiying,GONG Guoyi.Selection of Watermelon EST-SSR Molecular Marker[J].Journal of Changjiang Vegetables,2009(18):13-14. |
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| Authors: | ZOU Mingxue CHEN Yanhong XU Yong GUO Shaogui ZHANG Haiying GONG Guoyi |
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| Institution: | ZOU Mingxue1,CHEN Yanhong1,XU Yong2,GUO Shaogui2,ZHANG Haiying2,GONG Guoyi2 ( 1.Nantong University,Nantong,Jiangsu 226007,2.National Engineering Research Center for Vegetables ) |
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| Abstract: | From 820 ESTs of watermelon,we have identified 87 ESTs containing perfect simple sequence repeats (SSR) of di-,tri-,tetra-or pentanucleotides. 79 EST-SSR primer pairs were designed for watermelon based on the searching results. Through PCR amplifications and silver detection,32 primer pairs had successful and distinctive bands in different cultivars of watermelon. These EST-SSR markers can be taken as a new molecular marker of watermelon and related species. |
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| Keywords: | EST-SSR |
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