香蕉水通道蛋白基因在成熟果实中的表达分析与亚细胞定位研究

目的]更好了解香蕉果实成熟与水分代谢的关系。方法]用RT-PCR的方法探讨水通道蛋白基因MaPIP1;1在香蕉果实成熟过程中的表达情况,并利用绿色荧光融合蛋白研究MaPIP1;1的亚细胞定位。结果]香蕉水通道蛋白基因MaPIP1;1在正常和诱导成熟的果实中,表达持续下降。在高锰酸钾处理的果实中,MaPIP1;1在果实呼吸跃变发生之前表达平稳。亚细胞定位结果表明,MaPIP1;1主要定位于细胞质膜上。结论]推测MaPIP1;1基因在香蕉果实中可能起了运输水分或其他小分子物质的作用。

Research on the Expression of Banana Aquaporin Gene in Ripe Fruit and Sub-cellular Localization

Objective] The relationship between banana fruit maturity and water metabolism was better understood.Method] RT-PCR method was used to research the expression of aquaporin gene MaPIP1;1 in the process of banana fruit maturity and the MaPIP1;1 sub-cellular was located with the green fluorescent protein fusion.Results] The expression of banana aquaporin gene MaPIP1;1 was gradually reduced in normal/induced process of mature fruit.In the fruits treated with potassium permanganate,the MaPIP1;1 showed a smooth expression before respiratory-jumping.Sub-cellular localization showed the MaPIP1;1 was mainly posited on the cytoplasmic membrane.Conclusion] The MaPIP1;1 gene in banana fruit may play a water transport or other small molecules.