Up-regulated expression of PD-1 and its ligands during acute Classical Swine Fever virus infection in swine |
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| Authors: | Feng Yue Yan-Ping Zhu Yan-Fang Zhang Guo-Peng Sun Yuan Yang Dong-Guang Guo Ai-Guo Wang Bo-Wen Li Mei Yin An-Chun Cheng Ming-Shu Wang Xuan-Nian Wang |
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| Affiliation: | 1. Department of Life Sciense and Technology, Xinxiang University, Xinxiang, Henan 453003 China;2. College of Veterinary and Animal Sciences, Henan Istitute of Science and Technology, Xinxiang, Henan 453003, China;3. Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan 611130, China |
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| Abstract: | In order to investigate the relationship between the PD-1 pathway and impairment of immune responses with the CSFV infection, the mRNA expression of PD-1 and its ligands were evaluated by quantitative polymerase chain reaction (qPCR) during artificial CSFV infection. Simultaneously, expression of IL-2 and IL-10 mRNA were detected. The T cell proliferation and CSFV load in plasma were also measured. Results showed that the expression of PD-1 and its ligands mRNA were significantly increased (p < 0.01) in PBMC from 3 to 7 days post infection (dpi). Meanwhile the level of IL-10 was up-regulated (p < 0.01). The IL-2 mRNA was not obviously changed but it is significantly increased from 14 dpi. The T cell proliferation was notably decreased at 7 dpi. The CSFV load was also increased in plasma. Overall, our results suggest that the expression of PD-1 and its ligands were up-regulated and probably correlated with immune inhibition during acute CSFV infection. |
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| Keywords: | Classical swine fever virus Programmed death-1 Ligand Peripheral blood mononucleur cells Swine |
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