First finding of genetic and antigenic diversity in 1b-BVDV isolates from Argentina |
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| Authors: | A Pecora DA Malacari JF Ridpath MS Perez Aguirreburualde G Combessies AC Odeón SA Romera MD Golemba A Wigdorovitz |
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| Institution: | 1. Virology Institute, Veterinary and Agricultural Science Research Centre, INTA Castelar, Buenos Aires, Argentina;2. Ruminant Diseases and Immunology Research Unit USDA, Ames, USA;3. Laboratorio Azul Diagnóstico, Buenos Aires, Argentina;4. Laboratorio de Sanidad Animal, INTA Balcarce, Buenos Aires, Argentina;5. Departamento de Virología, Facultad de Farmacia y Bioquímica UBA, Buenos Aires, Argentina |
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| Abstract: | Infection with Bovine Viral Diarrhea Viruses (BVDV) in cattle results in a wide range of clinical manifestations, ranging from mild respiratory disease to fetal death and mucosal disease, depending on the virulence of the virus and the immune and reproductive status of the host. In this study 30 Argentinean BVDV isolates were characterized by phylogenetic analysis. The isolates were genotyped based on comparison of the 5′ untranslated region (5′ UTR) and the E2 gene. In both phylogenetic trees, 76% of the viruses were assigned to BVDV 1b, whereas BVDV 1a, 2a and 2b were also found. Eight of the BVDV 1b isolates were further characterized by cross-neutralization tests using guinea pig antisera and sera from bovines vaccinated with two different commercial vaccines. The results demonstrated the presence of a marked antigenic diversity among Argentinean BVDV isolates and suggest the need to incorporate BVDV 1b isolates in diagnostic strategies. |
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| Keywords: | Antigenic diversity Bovine Viral Diarrhea Virus Cattle viruses Phylogenetic analysis Pestivirus |
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