| 猪新基因NPM1的克隆、序列分析与染色体定位 |
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| 引用本文： | 唐中林,李勇,赵书红,刘榜,樊斌,朱猛进,李奎.猪新基因NPM1的克隆、序列分析与染色体定位[J].畜牧兽医学报,2007,38(2):105-109. |
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| 作者姓名： | 唐中林 李勇 赵书红 刘榜 樊斌 朱猛进 李奎 |
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| 作者单位： | 1. 华中农业大学农业动物遗传育种与繁殖教育部重点实验室,武汉,430070;中国农业科学院北京畜牧兽医研究所,北京,100094
2. 华中农业大学农业动物遗传育种与繁殖教育部重点实验室,武汉,430070 |
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| 基金项目： | 国家自然科学基金重点项目资助（30330440） |
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| 摘 要： | 克隆了猪NPM1基因的cDNA和第4内含子序列，并利用生物信息学方法进行验证。所得cDNA序列全长1195bp且包含一个完整的开放阅读框，编码294个氨基酸。内含子序列长300bp，遵循GT／AG剪接法则。序列分析表明，该基因与已报道的人、大鼠和小鼠等物种的NPM1基因高度同源，氨基酸序列同源性分别为95％、91％和91％。该基因编码的蛋白具有Nucleoplasmin保守功能域。用体细胞杂种板将该基因定位在猪16号染色体q21区段，辐射杂种板定位结果表明该基因与猪16号染色体上SW977标记紧密连锁。
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| 关 键 词： | 猪 NPM1基因 克隆 序列分析 染色体定位 |
| 文章编号： | 0366-6964（2007）02-0105-05 |
| 修稿时间： | 2006-03-02 |
Cloning, Sequence Analysis and Chromosome Mapping of Porcine New NPM1 Gene |
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| TANG Zhong-lin,LI Yong,ZHAO Shu-hong,LIU Bang,FAN Bin,ZHU Meng-jin,LI Kui.Cloning, Sequence Analysis and Chromosome Mapping of Porcine New NPM1 Gene[J].Acta Veterinaria et Zootechnica Sinica,2007,38(2):105-109. |
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| Authors: | TANG Zhong-lin LI Yong ZHAO Shu-hong LIU Bang FAN Bin ZHU Meng-jin LI Kui |
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| Institution: | 1. Key Laboratory of Agricultural Animal Heredity, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China ; 2. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China |
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| Abstract: | The cDNA and fourth intron sequences of porcine NPM1 gene were cloned,and verified by bioinformatics method.The cDNA fragment was 1 195 bp with an entire open reading frame(ORF),encoding 294 amino acids.The intron fragment was 300 bp and splice junctions follow 'GT/AG' rule.The analysis of sequence revealed that the porcine NPM1 gene was highly homologous with that of other species such as human(95%),mouse(91%) and rattus norvegicus(91%) in amino acid.The protein encoded by porcine NPM1 gene had a conserved domain 'Nucleoplasmin'.This gene was assigned to SSC16q21 region and closely linked to SW977 with the LOD of 9.16 by SCHP and IMpRH mapping,respectively. |
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| Keywords: | porcine NPM1 gene clone sequence analysis chromosome mapping |
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