| 猪伪狂犬病病毒的分离鉴定 |
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| 引用本文： | 韩伟,丁伯良,王英珍,张莉.猪伪狂犬病病毒的分离鉴定[J].天津农业科学,2009,15(3):4-6. |
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| 作者姓名： | 韩伟 丁伯良 王英珍 张莉 |
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| 作者单位： | 天津市畜牧兽医研究所,天津,300112 |
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| 基金项目： | 天津市科技发展计划项目，天津市应用基础及前沿技术研究计划 |
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| 摘 要： | 从天津市郊县某猪场发病仔猪脑中分离到一株病毒。该病毒接种鸡胚绒毛尿囊膜有白色的痘斑出现,接种PK-15和BHK-21细胞出现典型的细胞病变,毒价(TCID50)为10-6/mL,且能被PRV阳性血清中和;将病毒给家兔注射后出现典型的伪狂犬病症状并导致死亡。通过上述试验结果证明,该分离毒株为伪狂犬病毒。
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| 关 键 词： | 猪 伪狂犬病毒 分离 鉴定 |
Isolation and Identification of Swine Pseudorabies Virus |
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| HAN Wei,DING Bo-liang,WANG Ying-zhen,ZHANG Li.Isolation and Identification of Swine Pseudorabies Virus[J].Tianjin Agricultural Sciences,2009,15(3):4-6. |
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| Authors: | HAN Wei DING Bo-liang WANG Ying-zhen ZHANG Li |
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| Institution: | (Tianjin Institute of Animal Husbandry and Veterinary Science, Tianjin 300112, China) |
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| Abstract: | One virus was isolated from brain organ of diseased pig which came from a pig farm in Tianjin. After inoculating the virus, the white spots could be seen in chick embryo chorioallantoic membrane, and typical cytopathogenic effect（CPE）appeared in PK-15 and BHK-21 cells. TCIDs0 of the virus was lO-6/mL.The virus could be neutralized by standard positive serum of pseudorabies virus. After injecting the virus, rabbits showed typical pseudorables clinical symptom and died. By the above experiment results, the isolated virus was identified as PRV. |
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| Keywords: | pig pseudorabies virus isolation identification |
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