23个广西本地芒果品种的ISSR分析

本研究对23个分别来自广西本地品种或广西选育的品种进行了SSR分析,并用15个芒果栽培品种印度（4个品种）、美国（3个品种）、缅甸（3个品种）、泰国（2个品种）及菲律宾、巴基斯坦和斯里兰卡（各1个品种）和2个芒果近源种冬芒（1个）和扁桃（1个）作为参照.从42个ISSR引物中筛选出9个多态性好的引物构建DNA指纹图谱来鉴别芒果的基因型.根据这9个引物获得的电泳图谱结果表明,本研究中所有供试品种能互相区分开来并表现出丰富的遗传多样性,说明ISSR是一种鉴定芒果品种（系）非常有效的方法.用74条多态性条带聚类分析作图发现所有供试的广西品种与秋芒、马切苏芒、象牙芒和爱文芒归为一个大类,而且广西本地芒果品种之间的亲缘关系较近.

Genetic Analysis of 23 Mango Cultivar Collection in Guangxi Province Revealed by ISSR

Twenty-three mango cultivars (Mangifera indica Linn) collecting in Gua ngxi province were examined by ISSR markers and two species relatives M. himalis J.Y. Liang and M. persiciformis Wu & Ming, other 15 mango cultivars from India (4), USA (3), Burma (3), Thailand (2), Philippines (1), Pakistan (1) and Sir La nka (1) used as references. Of the 42 primers screened, 9 primers gave reproduci ble, polymorphic DNA amplification patterns, and were selected to construct a DN A fingerprinting map to distinguish the genotypes of mango. According to the ban ding patterns obtained with 9 selected primers, all cultivars tested in this stu dy were distinguished from each other and showed ample genetic diversity, indica ting that ISSR-PCR was an effective method for cultivar identification of mangoe s. Based on 74 selected bands, all Guangxi mango cultivars tested were clustered into a big group with Neelum, Macheso, Aroemwnis, and Irwin by UPGMA analysis, indicating that Guangxi mango cultivars had a close relationship each other.