| CSFV免疫状态下外周血T淋巴细胞亚群变化及对PRRS的免疫保护作用 |
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| 引用本文： | 屈雪琪,白雪,王异民,赵建增,高英杰.CSFV免疫状态下外周血T淋巴细胞亚群变化及对PRRS的免疫保护作用[J].中国兽医学报,2011,31(8):1089-1094. |
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| 作者姓名： | 屈雪琪 白雪 王异民 赵建增 高英杰 |
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| 作者单位： | 1. 吉林大学畜牧兽医学院,林长春,130062
2. 北京必威安泰生物科技有限公司,北京,100176 |
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| 基金项目： | 国家自然科学基金资助项目(30972193) |
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| 摘 要： | 建立CSFV疫苗免疫状态下猪繁殖与呼吸综合征病毒（PRRSV）感染动物模型。测定CSFV抗体阻断率和PRRSV抗体S/P值,根据CSFV抗体阻断率和PRRSV感染情况分析猪外周血中T淋巴细胞亚群变化和对PRRS的保护促进作用。采用流式细胞术（FAC）检测猪外周血T淋巴细胞CD3、CD4、CD8亚群的变化。结果,CSFV...
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| 关 键 词： | 猪瘟疫苗 猪繁殖与呼吸综合征病毒 流式细胞术 淋巴细胞亚群 保护作用 |
The changes of peripheral blood T lymphocyte subpopulations and the protection for PRRS of pigs vaccinated with CSFV vaccine |
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| QU Xue-qi,BAI Xue,WANG Yi-min,ZHAO Jian-zeng,GAO Ying-jie.The changes of peripheral blood T lymphocyte subpopulations and the protection for PRRS of pigs vaccinated with CSFV vaccine[J].Chinese Journal of Veterinary Science,2011,31(8):1089-1094. |
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| Authors: | QU Xue-qi BAI Xue WANG Yi-min ZHAO Jian-zeng GAO Ying-jie |
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| Institution: | QU Xue-qi1,BAI Xue1,WANG Yi-min1,ZHAO Jian-zeng2,GAO Ying-jie1(1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China,2.Beijing Bigvet Biotech CO.LTD,Beijing 100176) |
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| Abstract: | Pigs which vaccinated with classical swine fever virus vaccine were used to the changes of peripheral blood T lymphosyte subpopulations and the protection for PRRS,CSFV and PRRSV antibody in pigs was detected by Blocking ELISA of IDEXX company and LISTEST SUIS PRRS,and grouped according to test results.The dynamic changes of CD3,CD4,CD8 T lymphocyte subpopulation in peripheral blood were analyzed using flow cytometry(FC).The results showed that the in infected PRRSV ratio were high-CSFV antibody groups high... |
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| Keywords: | CSFV vaccine PRRSV flow cytometry lymphocyte subpopulation protection |
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