| H5-H7双价禽流感核酸疫苗免疫研究 |
| |
| 引用本文： | 张丹,鲁会军,谭磊,刘存霞,胡博,刘燕瑜,刘昊,金扩世,金宁一.H5-H7双价禽流感核酸疫苗免疫研究[J].中国兽医学报,2011,31(2). |
| |
| 作者姓名： | 张丹 鲁会军 谭磊 刘存霞 胡博 刘燕瑜 刘昊 金扩世 金宁一 |
| |
| 作者单位： | 吉林大学畜牧兽医学院;解放军军事医学科学院军事兽医研究所全军基因工程重点实验室; |
| |
| 基金项目： | “863”重大项目(2006AA10A205); 国家科技支撑项目(2006BAD06A05) |
| |
| 摘 要： | 由于禽流感的高度变异性,免疫后不同亚型之间难以得到交叉保护,以致病毒得以逃避宿主免疫系统的监视,多价核酸禽流感疫苗具有可以保护不同亚型病毒攻击的特点。本试验设计并构建了包含禽流感H5HA和H7HA1基因的双价真核表达质粒pV-H5-H7及单独表达H5HA和H7HA1的pV-H5和pV-H7HA1。通过RT-PCR,间接免疫荧光(IFA)等方法验证构建质粒的正确性和其表达蛋白的免疫原性。0,21 d分别免疫6~8周龄的BALB/c小鼠,设立双价疫苗组,单表达免疫组和对照组。免疫后35 d用HPAIV H5N1进行致死性攻击。结果显示免疫组均可刺激机体产生H5特异性抗体,pV-H5-H7诱导产生的抗体对H5N1的攻毒保护率为80%,而pV-H5单表达的攻毒保护率也为80%。表明本实验构建的双价禽流感核酸疫苗的免疫效果与单表达组相当(P>0.05),为多价禽流感核酸疫苗的研制提供了基础。
|
| 关 键 词： | 禽流感 H5 H7亚型 核酸疫苗 免疫攻毒 |
Study on construction and experimental immunity of H5,H7 subtype influenza virus bivalent DNA vaccine |
| |
| ZHANG Dan,LU Hui-jun,TAN Lei,LIU Cun-xia,HU Bo,LIU Yan-yu,LIU Hao,JIN Kuo-shi,JIN Ning-yi.Study on construction and experimental immunity of H5,H7 subtype influenza virus bivalent DNA vaccine[J].Chinese Journal of Veterinary Science,2011,31(2). |
| |
| Authors: | ZHANG Dan LU Hui-jun TAN Lei LIU Cun-xia HU Bo LIU Yan-yu LIU Hao JIN Kuo-shi JIN Ning-yi |
| |
| Institution: | ZHANG Dan1,2,LU Hui-jun2,TAN Lei1,LIU Cun-xia1,HU Bo1,LIU Yan-yu1,LIU Hao1,JIN Kuo-shi2,JIN Ning-yi2(1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China,2.Institute of Military Veterinary,Academy of Military Medical Science,China) |
| |
| Abstract: | It is difficult to provide cross-protection among different subtypes after immunization because of avian influenza virus with high variability,which cause the virus could escape the host immune surveillance.Multivalence DNA vaccine of avian influenza may protect animals from different subtypes.This experimental design was constructed to contain the avian influenza virus H5HA and H7HA1 bivalent-gene plasmid pV-H5-H7 and single-gene H7HA1,H5HA named pV-H5 and pV-H7HA1.By RT-PCR and indirect immunofluorescence... |
| |
| Keywords: | avian flu H5 H7 subtypes nucleic acid vaccines virus challenge |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
