利用DH和IF_2群体检测油菜籽粒油酸、亚油酸、亚麻酸含量QTL

以德国冬性甘蓝型油菜Express和中国半冬性甘蓝型油菜SWU07为亲本构建包含261个株系的DH群体和包含234个株系的IF2群体,检测不同年份条件下油菜籽粒油酸、亚油酸、亚麻酸含量相关的QTL。在DH群体4年环境下共检测出71个QTL,在IF2群体2年环境下共检测出4个QTL。去掉在不同年份和群体中置信区间相互重叠的QTL之后,共得到3个品质性状的51个QTL,其中有15个在2年以上环境中被检测到。这些QTL分别分布在13个连锁群上,其中与油酸含量相关的18个,分布于A01、A02、A04、A05、A07、A09、C01连锁群,揭示3.44%～13.97%的表型变异;与亚油酸相关的12个,分布于A02、A06、A09、C01、C02连锁群,揭示3.84%～19.51%的表型变异;与亚麻酸相关的21个,分布于A01、A02、A03、A04、A05、A08、A09、C01、C02、C03、C06连锁群,揭示2.86%～11.91%的表型变异。这些结果将为油菜脂肪酸品质改良提供更多遗传信息。

QTL mapping of oleic acid,linoleic acid and linolenic acid contents in Brassica napus L. using DH and IF2 populations

DH and IF₂ populations, consisting of 261 and 234 lines respectively, constructed using the German winter rapeseed cultivar ‘Express’ (female) and the Chinese semi-winter line ‘SWU07’ (male), were used to detect QTLs related to oleic acid, linoleic acid and linolenic acid contents in different years. A total of 71 QTLs were detected in DH population in four-year environment and 4 QTLs in IF₂ population in two-year environment. After eliminating the overlapped QTLs detected in different years or different populations, totally 51 QTLs for the three traits were obtained, 15 QTLs of which were persistently detected in more than two years. These 51 QTLs were located on 13 linkage groups. Among them, 18 QTLs related to oleic acid content were located on A01, A02, A04, A05, A07, A09, and C01, respectively, explaining phenotypic variation ranging from 3.44% to 13.97%, 12 QTLs related to linoleic acid content were located on A02, A06, A09, C01, and C02, respectively, explaining phenotypic variation ranging from 3.84% to 19.51%, and 21 QTLs related to linolenic acid content were located on A01, A02, A03, A04, A05, A08, A09, C01, C02, C03, and C06, respectively, explaining phenotypic variation from 2.86% to 11.91%. These results provide more genetic information for the improvement of fatty acid quality in rapeseed breeding.