| 不同剂量PMSG/HCG对小鼠超数排卵的效果 |
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| 引用本文： | 冯雨峰,闫雷.不同剂量PMSG/HCG对小鼠超数排卵的效果[J].家畜生态,2008(4). |
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| 作者姓名： | 冯雨峰 闫雷 |
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| 作者单位： | 中国环境管理干部学院;河北科技师范学院; |
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| 摘 要： | 确定昆明种小白鼠超排的最佳激素剂量和分析其他影响超排的因素。将40只雌性昆明小白鼠随机分为四组。每组注射PMSG和HCG分别为5IU+5IU、5IU+10IU、10IU+5IU和10IU+10IU,注射间隔时间为48h,比较排出卵母细胞的数量。结果表明,试验3组小鼠排出卵母细胞的平均数量(57.1枚),显著(P<0.05)高于试验1组(42.7枚)和试验2组(41.7枚),极显著(P<0.01)高于试验4组(30.7枚)。其它三组间无显著差异。左右卵巢的排卵数也未表现出明显差异。初步说明昆明小白鼠超排的最佳激素剂量为10IU PMSG+5IU HCG,但要想得到很好的超排效果还要考虑其他因素。
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| 关 键 词： | 小鼠 超数排卵 PMSG HCG |
Effect of PMSG/HCG Dosages on Superovulation in Mice |
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| FENG Yu-feng,YAN Lei.Effect of PMSG/HCG Dosages on Superovulation in Mice[J].Ecology of Domestic Animal,2008(4). |
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| Authors: | FENG Yu-feng YAN Lei |
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| Institution: | FENG Yu-feng1,YAN Lei2(1.Environmental Management College of China,Qinhuangdao Hebei 066004,2.Hebei Normal University of Science & Technology,Qinhuangdao Hebei 066004) |
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| Abstract: | Objective:This study was carried out to determine the optimum hormone dosage for the superovulation of Kunming mice.Method:A total of 40 female Kunming mice were divided randomly into four groups,and treated at the interval of 48h wih 5IU+5IU,5IU+10IU,10IU+5IU,or 10IU+10IU PMSG and HCG to stimulate superovulation of Kunming mice.Result:The average number of ovocyte in group 3 was significantly higher(P<0.05) than group 1 and group 2,as well as group 4(P<0.01).There were no differences(P>0.05) among other th... |
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| Keywords: | mice superovulation PMSG HCG |
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