| 华盖木多酚氧化酶酶学特性研究 |
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| 引用本文： | 马均,马明东.华盖木多酚氧化酶酶学特性研究[J].福建林业科技,2009,36(1). |
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| 作者姓名： | 马均 马明东 |
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| 作者单位： | 四川农业大学都江堰分校,四川,都江堰,611830 |
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| 基金项目： | 四川农业大学都江堰分校科技基金，四川省科技厅应用基础研究项目 |
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| 摘 要： | 以华盖木嫁接苗当年生茎段为试材提取PPO酶液,研究温度、pH值、底物邻苯二酚浓度及抑制物对PPO活性的影响。结果表明:华盖木多酚氧化酶的最适温度为35℃,最适pH值为7.0,温度和pH值过高或过低都会降低酶活性;邻苯二酚浓度为0.14 mo.lL-1时,PPO活性最大,低于0.14 mo.lL-1时,随底物浓度的增加,酶活性增加,超过0.14 mol.L-1时,加大底物浓度酶活性降低;Na2SO4、柠檬酸、抗坏血酸(Vc)对华盖木PPO的活性均有一定的抑制效果,其中Vc的抑制效果最显著。
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| 关 键 词： | 华盖木 多酚氧化酶 酶学特性 |
Study on the Enzymology Characteristics of Plyphenol Oxidase from Manglietiastrum sinicum Law. |
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| MA Jan,MA Ming-dong.Study on the Enzymology Characteristics of Plyphenol Oxidase from Manglietiastrum sinicum Law.[J].Journal of Fujian Forestry Science and Technology,2009,36(1). |
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| Authors: | MA Jan MA Ming-dong |
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| Institution: | MA Jun,MA Ming-dong(Dujiangyan Branch of Sichuan Agriculture University,Dujiangyan 611830,Sichuan,China) |
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| Abstract: | PPO was extracted from the Manglietiastrum sinicum Law grafted plant stem before it was 1 year old.The influences of different temperature、pH、zymolyte oxopenic acid concentration and stayer on the activity of PPO were studied.The results showed that the optimal temperature was 35℃ and the optimal pH value was 7.0 for plyphenol oxidase from M.sinicum Law;The activity of PPO would reduce because of elevating or reducing temperature or pH.The PPO activity was highest when oxopenic acid concentration was 0.14 mol·L-1.Under the concentration,the enzyme activity increased with zymolyte concentration increase.Over the concentration,the encyme activity decreased;Na2SO4、citric acid and Vc could suppress the activity of PPO,among them,Vc possesed the most remarkable controlling effect. |
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| Keywords: | Manglietiastrum sinicum Law polyphenol oxidase enzymology characteristics |
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