| 玉米淀粉分支酶SBEIIb基因遗传转化玉米自交系的研究 |
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| 引用本文： | 车昕明,郭新梅,裴玉贺,高学艳,宋希云.玉米淀粉分支酶SBEIIb基因遗传转化玉米自交系的研究[J].华北农学报,2011(5):50-53. |
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| 作者姓名： | 车昕明 郭新梅 裴玉贺 高学艳 宋希云 |
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| 作者单位： | 青岛农业大学生命科学学院;青岛农业大学青岛市主要农作物种质资源创新与应用重点实验室;青岛农业大学农学与植保学院; |
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| 基金项目： | 山东省良种产业化工程; 青岛市自然科学基金(07-2-3-6-jch); 青岛市农业科技支撑项目(09-1-1-70-nsh) |
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| 摘 要： | 以玉米自交系丹黄25为试材,用农杆菌介导法将玉米淀粉分支酶SBEIIb基因转入玉米中,探讨了农杆菌介导玉米愈伤组织转化的影响因素.结果表明:愈伤组织经过9d的继代,菌液中乙酰丁香酮(AS)浓度为10 mg/L,侵染时间为20 min,共培养60 h为最佳转化条件.获得的4株再生植株经PCR分析鉴定,其中3株表现阳性,证...
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| 关 键 词： | 玉米 农杆菌介导 转化条件 SBEIIb基因 |
Study on Transformation of Starch Branching Enzyme SBEIIb Gene into Maize Inbred Line |
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| CHE Xin-ming,GUO Xin-mei,PEI Yu-he,GAO Xue-yan,SONG Xi-yun.Study on Transformation of Starch Branching Enzyme SBEIIb Gene into Maize Inbred Line[J].Acta Agriculturae Boreali-Sinica,2011(5):50-53. |
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| Authors: | CHE Xin-ming GUO Xin-mei PEI Yu-he GAO Xue-yan SONG Xi-yun |
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| Institution: | CHE Xin-ming1,3,GUO Xin-mei2,PEI Yu-he2,GAO Xue-yan2,SONG Xi-yun1(1.College of Life Science,Qingdao 266109,China,2.College of Agronomy and Plant Protection,3.Qingdao Key Lab of Germplasm Innovation and Application of Major Crops,Qingdao Agricultural University,China) |
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| Abstract: | The SBEIIb gene was transferred into maize inbred line Danhuang 25 by Agrobacterium tumefaciens mediated transformation.Factors influenced transformation efficiency was studied.The results showed that 9 days with subculture of callus,AS concentration in the bacilli concentration at 10 mg/L,20 minutes infection time and co-culture 60 hours were the optimal conditions.PCR analysis on four resistance plants demonstrated that three of them were positive and the foreign gene was integrated into maize genome. |
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| Keywords: | Maize Agrobacterium tumefaciens mediated Conversion conditions SBEIIb gene |
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