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番茄果实EST资源SSR信息分析
引用本文:韩明利,崔娜,于志海,李天来,侯丽霞.番茄果实EST资源SSR信息分析[J].华北农学报,2011,26(4):213-217.
作者姓名:韩明利  崔娜  于志海  李天来  侯丽霞
作者单位:1. 沈阳农业大学生物科学技术学院,辽宁沈阳,110866
2. 沈阳农业大学生物科学技术学院,辽宁沈阳110866;沈阳农业大学园艺学院,设施园艺重点实验室,辽宁沈阳110866
3. 沈阳农业大学园艺学院,设施园艺重点实验室,辽宁沈阳110866
4. 山东农业科学院蔬菜所,山东济南,250100
基金项目:岗位专家课题资助项目:国家现代农业产业体系建设专项(Nycytx-35-gw23); 国家“863”计划项目(2006AA100108-3-1); 辽宁省教育厅科学技术研究项目(2008623); 沈阳农业大学博士后基金项目(62658)
摘    要:表达序列标签( Expressed sequence tags,EST)中存在广泛的微卫星或简单重复序列(Simple sequence repeats,SSR),为SSR标记的开发提供了宝贵的资源.以NCBI中与番茄(Solanum lycopersicum)果实性状相关EST序列为材料,对EST序列进行聚类去冗余、...

关 键 词:番茄  EST-SSR  果实

Analysis of SSR Information in EST Resource of Tomato (Solanum lycopersicum) Fruit
HAN Ming-li,CUI Na,YU Zhi-hai,LI Tian-lai,HOU Li-xia.Analysis of SSR Information in EST Resource of Tomato (Solanum lycopersicum) Fruit[J].Acta Agriculturae Boreali-Sinica,2011,26(4):213-217.
Authors:HAN Ming-li  CUI Na  YU Zhi-hai  LI Tian-lai  HOU Li-xia
Institution:HAN Ming-li1,CUI Na1,2,YU Zhi-hai1,LI Tian-lai2,HOU Li-xia3(1.Biological Science and Technology College,Shenyang Agricultural University,Shenyang 110866,China,2.College of Horticulture,Key Laboratory of Protected Horticulture,Ministry of Education,3.Vegetable Research Institute of Shandong Academy of Agricultural Sciences,Jinan 250100,China)
Abstract:Expressed sequence tags(ESTs)are important resources for development of new SSR markers.83 613 ESTs of the tomato fruit in the database of NCBl were downloaded and early treatmented,such as being clusterd to redundancy and spliced,and so on.It is concluted that there were 18 878 UniGene after being spliced by CAP3 tool,in which of 9 650 Contigs and 9 228 Singlets.2 039 EST-SSRs were gotten by using MISA tool searched SSR,accounting for 12.62% of the UniGene and including 97 repeat motifs.Mononucleotide,Dinu...
Keywords:Tomato  EST-SSR  Fruit  
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