| 小麦NBS类抗病基因类似序列的多样性和进化关系研究 |
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| 引用本文： | 张立荣,杨文香,刘大群. 小麦NBS类抗病基因类似序列的多样性和进化关系研究[J]. 华北农学报, 2011, 26(4): 23-26 |
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| 作者姓名： | 张立荣 杨文香 刘大群 |
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| 作者单位： | 河北农业大学植物保护学院植物病理系,河北省农作物病虫害生物防治工程技术研究中心,河北保定071001 |
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| 基金项目： | 国家自然科学基金项目(30771391); 河北省青年基金项目(2010140) |
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| 摘 要： | 利用已克隆植物抗病基因NBS( Nucleotide binding site)序列中的保守结构P-loop和GLPL合成简并引物,以小麦近等基因系TcLr24基因组DNA为模板进行PCR扩增.得到13条具有连续ORF的抗病基因类似物(Resistance gene analogues,RGAs)序列,它们之间相应推测...
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| 关 键 词： | 小麦 NBS 抗病基因类似物 |
Diversity and Evolutionary Relationship of NBS-type Resistance Gene Analogues in Wheat |
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| ZHANG Li-rong,YANG Wen-xiang,LIU Da-qun. Diversity and Evolutionary Relationship of NBS-type Resistance Gene Analogues in Wheat[J]. Acta Agriculturae Boreali-Sinica, 2011, 26(4): 23-26 |
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| Authors: | ZHANG Li-rong YANG Wen-xiang LIU Da-qun |
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| Affiliation: | ZHANG Li-rong,YANG Wen-xiang,LIU Da-qun(College of Plant Protection,Biological Control Center of Plant Diseases and Pests of Hebei Province,Agricultural University of Hebei,Baoding 071001,China) |
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| Abstract: | Degenerate primers based on conserved motif(P-loop and GLPL)of the nucleotide binding site(NBS) region from the cloned plant disease resistance genes were used to isolate resistance gene analogues(RGAs) from genomic DNA of wheat.13 RGAs with uninterrupted open reading frames(ORFs) were obtained.Sequence identity among the 13 RGAs deduced amino acid sequences showed identity ranged from 36.9 % to 98.3%.The analysis of RGAs amino acid sequence structures suggested that they contained the domains such as P-loo... |
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| Keywords: | Wheat NBS(Nucleotide binding site) RGA(Resistance gene analogues) |
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