| 兰州百合鳞片组织培养研究 |
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| 引用本文： | 刘雅楠,黄惠英,张金文.兰州百合鳞片组织培养研究[J].草原与草坪,2010,30(4):26-30. |
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| 作者姓名： | 刘雅楠 黄惠英 张金文 |
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| 作者单位： | 1. 甘肃农业大学,植物生产类实验教学中心,甘肃,兰州,730070
2. 甘肃农业大学,植物生产类实验教学中心,甘肃,兰州,730070;甘肃农业大学,农学院,甘肃,兰州,730070 |
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| 基金项目： | 甘肃省科技厅支撑计划项目"兰州百合优良品种培育和母籽脱毒技术开发" |
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| 摘 要： | 以兰州百合的内层鳞片为外殖体,以MS为基本培养基并添加不同配比生长调节剂,进行组织培养研究。结果表明,以MS+1.0 mg/L 6-BA+0.2 mg/L NAA培养基对初代培养形成不定芽最佳,低温有助于外殖体分化;不定芽增殖最适培养基为MS+0.5 mg/L 6-BA+0.5 mg/L NAA;1/2 MS+0.2 mg/L NAA+蔗糖60 g/L适宜苗的生根培养,生根率达100%,且结鳞茎率达73.4%。
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| 关 键 词： | 兰州百合 鳞片 消毒 组织培养 |
Tissue culture of Lilium davidii var.willmottiae flakes in Lanzhou |
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| LIU Ya-nan,HUANG Hui-ying,ZHANG Jing-wen.Tissue culture of Lilium davidii var.willmottiae flakes in Lanzhou[J].Grassland and Turf,2010,30(4):26-30. |
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| Authors: | LIU Ya-nan HUANG Hui-ying ZHANG Jing-wen |
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| Institution: | LIU Ya-nan,HUANG Hui-ying,ZHANG Jing-wen(1.Experimental Center for Plant Production,Gansu Agricultural University,Lanzhou 730070,China;2.College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China) |
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| Abstract: | The inner flakes of lily were used as explants to induce the additional shoots in MS medium with different hormone combinations,and the effects of hormones and temperature on the induction of shoots were studied.The results showed that the best culture medium for the induction and differentiation of addition shoot was MS ＋ 1.0 mg/L 6-BA ＋ 0.2 mg/L NAA in low temperature;the best medium in the stage of shoots multiplications was MS ＋ 0.5 mg/L 6-BA ＋ 0.5 mg/L NAA;and 1/2 MS ＋ 0.2 mg/L NAA ＋ sucrose 60 g/L was the most effective media for rooting inducement,its rooting rate was 100% and the bulb rate was 73.4%. |
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| Keywords: | Lilium davidii var willmottiae flake sterilization tissue culture |
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