兔抗鸡传染性法氏囊病抗体的制备和纯化

采用自然发病的传染性法氏囊病（IBD）病鸡的法氏囊组织制作鸡IBD油佐剂灭活苗，用鸡IBD油佐剂灭活苗多点皮下注射健康成年兔，获得高免兔抗鸡IBDV血清，经过盐析、透析的方法制备高纯度的兔抗鸡IBDV高免IgG。兔血清中抗体效价为1∶128，蛋白质含量为40.3~48.8 mg/ml；纯化后效价为1∶32，蛋白质含量为18.0~23.0 mg/ml。用此试剂对安徽部分地区疑似鸡IBD的病例进行了诊断，取得良好效果。该试剂为临床琼脂扩散试验(AGP)检测IBDV抗原提供高效价的抗体，并为临床免疫组化法检测IBDV提供可靠的抗体。

Preparation and Purification of Rabbit Anti-Infectious Bursal Disease Antibody

Fowl infectious bursal disease(IBD) eleo adjuvant deactivation vaccine was prepared from bursa of sick chick. And it was used to immune health and adult rabbit by multidrop hypodermic injection to get rabbit anti IBDV serum. Hypso exempt IgG was isolated and purified from the serum. The antibody titer was 1∶128 in rabit serums, the protein contents were 40.3 to 48.8 mg/ml; The antibody titer was 1∶32 after purification, the protein contents were 18.0 to 23.0 mg/ml. Cases of doubtful fowl IBD in several regions of Anhui province were effectively diagnosised with this agent. It provided high titer antibody to detect IBDV antigen in AGP and immune histochemistry.