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蒽酮-硫酸法测定芝芪菌质中的多糖含量
引用本文:阮鸣,韩亚东.蒽酮-硫酸法测定芝芪菌质中的多糖含量[J].安徽农业科学,2008,36(27).
作者姓名:阮鸣  韩亚东
作者单位:南京晓庄学院生命科学系药用菌物研究所,江苏南京,211171
摘    要:目的]用蒽酮-硫酸法测定芝芪菌质中的多糖含量。方法]研究确定蒽酮-硫酸法测定芝芪菌质中多糖含量的最佳检测条件,测定3批芝芪菌质的多糖含量。结果]当加入6 ml、0.05%的硫酸蒽酮溶液,在水浴中加热20 min,冷却20 min时芝芪菌质中多糖含量测定条件最优。结论]3批芝芪菌质多糖的平均含量为0.991 3%。

关 键 词:芝芪菌质  多糖  蒽酮-硫酸法  含量测定

Content Determination of Polysaccharide in Zhi Qi Fungal Substance by Anthrone-sulfate Method
RUAN Ming et al.Content Determination of Polysaccharide in Zhi Qi Fungal Substance by Anthrone-sulfate Method[J].Journal of Anhui Agricultural Sciences,2008,36(27).
Authors:RUAN Ming
Abstract:Objective] The research aimed to determine the content of polysaccharide in Zhi Qi fungal substance by anthrone-sulfate method.Method] The optimum determination conditions of polysaccharide content in Zhi Qi fungal substance by anthrone-sulfate method were confirmed and polysaccharide content in 3 batches of Zhi Qi fungal substance was determined.Result] The optimum determination conditions for polysaccharide content in Zhi Qi fungal substance were as follows: adding 6 ml 0.05% anthrone-sulfate solution,heating for 20 min in water bath and cooling for 20 min.Conclusion] The average content of polysaccharide in 3 batches of Zhi Qi fungal substance was 0.991 3%.
Keywords:Zhi Qi fungal substance  Polysaccharide  Anthrone-sulfate method  Determination
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