| 12个无芒雀麦种群遗传多样性的RAPD分析 |
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| 引用本文： | 张凤霞,王铁娟,王照兰,杜建材,田青松,吴新宏. 12个无芒雀麦种群遗传多样性的RAPD分析[J]. 中国草地学报, 2011, 33(2) |
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| 作者姓名： | 张凤霞 王铁娟 王照兰 杜建材 田青松 吴新宏 |
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| 作者单位： | 1. 内蒙古师范大学生命科学与技术学院,内蒙古,呼和浩特,010022
2. 中国农业科学院草原研究所/农业部草原资源与生态重点开放实验室,内蒙古,呼和浩特,010010 |
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| 基金项目： | 国家草品种区域试验项目，国家科技支撑计划课题，中央级公益性科研院所基本科研业务费专项资金(中国农业科学院草原研究所)资助项目 |
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| 摘 要： | 用RAPD分子标记对12个无芒雀麦种群进行遗传多样性分析,结果表明:选用的9条多态性引物共扩增出87个条带,平均每个引物扩增9.67条,其中多态性条带45个,多态性条带百分率为51.72%.无芒雀麦物种水平上的多态性条带数为76个,多态性条带百分率为87.36%,Shannon信息指数为0.2933,Nei s基因多样性指数为0.1843.种群间遗传分化系数为0.2735,说明无芒雀麦的遗传分化主要发生在种群内.聚类结果显示,部分种群间的遗传距离与地理距离有一定的相关性,但也有例外,这可能与人类的广泛栽培加大了种群间的基因交流有关.
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| 关 键 词： | 无芒雀麦 种群 RAPD 遗传多样性 |
Genetic Diversity Analysis of 12 Bromus inermis Leyss Populations Based on RAPD Markers |
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| ZHANG Feng-xia,WANG Tie-juan,WANG Zhao-lan,DU Jian-cai,TIAN Qing-song,WU Xin-hong. Genetic Diversity Analysis of 12 Bromus inermis Leyss Populations Based on RAPD Markers[J]. Chinese Journal of Grassland, 2011, 33(2) |
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| Authors: | ZHANG Feng-xia WANG Tie-juan WANG Zhao-lan DU Jian-cai TIAN Qing-song WU Xin-hong |
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| Affiliation: | ZHANG Feng-xia1,WANG Tie-juan1,WANG Zhao-lan2,DU Jian-cai2,TIAN Qing-song2,WU Xin-hong2(1.Science and Technology College,Inner Mongolia Normal University,Hohhot 010022,China,2.Grassland Research Institute,Chinese Academy of Agricultural Science/Key Laboratory of Grassland Resources & Ecology,Ministry of Agriculture,Hohhot 010010,China) |
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| Abstract: | Random amplified polymorphic DNA(RAPD) techniques were used to detect the genetic diversity of 12 Bromus inermis Leyss populations.A total of 87 bands were amplified with 9 RAPD primers,of which 45 were polymorphic bands.The average percentage of polymorphic bands were 51.72%.76 were polymorphic(PPB=87.36%) in species level.The results of Popgen32 analysis indicated that Nei's gene diversity(H) was 0.1843,Shannon information index(I*) was 0.2933.Gene difference index(Gst) among populations was 0.2735,which ... |
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| Keywords: | Bromus inermis Leyss Population RAPD Genetic diversity |
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