| 托柄菝葜组织培养技术研究 |
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| 引用本文： | 刘永提,黄成名,姚东艳,李林,胡明芬.托柄菝葜组织培养技术研究[J].林业科技开发,2006,20(3):42-45. |
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| 作者姓名： | 刘永提 黄成名 姚东艳 李林 胡明芬 |
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| 作者单位： | 1. 宜昌市林业科学研究所
2. 宜昌市夷陵区林业局 |
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| 摘 要： | 以托柄菝葜幼嫩的茎段为外植体,MS、1／2MS、1／4MS、B5为基本培养基,研究了不同植物激素组合对托柄菝葜组织培养快速繁殖的影响。试验结果表明,不同培养阶段的适宜培养基为:(1)启动培养基为1／2MS 6-BA 2．0 mg／L IBA 0．1 mg／L;(2)继代增殖培养基为1／2MS 6-BA 3．0 mg／L IBA 0．2 mg／L,月平均芽增殖率可达 177．8％;(3)生根培养基为1／4MS NAA 1．5 mg／L 活性炭1 000 mg／L,培养25 d后生根率可达93．3％。
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| 关 键 词： | 托柄菝葜 组织培养 茎段 外植体 褐化 |
| 收稿时间： | 2006-03-13 |
| 修稿时间： | 2006-03-14 |
Tissue Culture of Smilax discotis Warb |
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| LIU Yong-ti,HUANG Cheng-ming,YAO Dong-yan,LI Lin,HU Ming-fen.Tissue Culture of Smilax discotis Warb[J].China Forestry Science and Technology,2006,20(3):42-45. |
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| Authors: | LIU Yong-ti HUANG Cheng-ming YAO Dong-yan LI Lin HU Ming-fen |
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| Abstract: | Taking the young stem of SmUax discotis Warb. as the explant, using the MS,1/2MS,1/4MS,B5 as the basic culture medium, the effects of hormone combination on callus formation and plantlet regeneration of SmUax discotis Warb. were studied in this paper. The results indicated that the suitable culture medium at different culture stages was: (1)Initial medium: 1/2MS 6 -BA2.0mg/L IBA0.1mg/L;(2)Proliferation medium: 1/2MS 6- BA3.0mg/L IBA0.2mg/L,(3)Rooting medium: 1/4MS NAA1.5mg/L activated carbon 1 000mg/L. The monthly average bud proliferation rate could reach 177.8% in proliferation medium, and the rooting ratio could reach 93.3% when cultivated in rooting medium for 25d. |
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| Keywords: | Smite discotis Warb Tissue culture Stem segment Explant Browning |
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