一株海洋细菌HZBN43的鉴定

[目的]鉴定1株分离自黄海海水的细菌菌株HZBN43。[方法]采用16S rRNA技术,对细菌样品株HZBN43进行鉴定,并对其进行系统分析。[结果]通过PCR扩增获得1 521 bp的16S rRNA序列,将其与NCBI已登陆的其他同源序列进行系统分析,确定了此株海洋细菌属于芽孢杆菌属(Bacillus)。虽然与已知种Bacillus selenatarsenatis的相似性为99.79%,但系统分析表明,置信度仅为46,可能受分析序列较短所限。要将其确定到种,尚需要其他研究佐证。

Preliminary Identification of a Marine Bacterial Strain HZBN43

[Objective] The aim of this study is to identify a bacterial strain isolated from ocean water from the Yellow Sea.[Method] Using 16S rRNA technique, a strain from Yellow Sea was preliminarily identified and analyzed.[Result] A 1521 bp fragment of 16S rRNA was amplified from the strain HZBN43;homology analysis between the yielded sequence and the 16S rRNA sequences accessed in NCBI from other strains showed that HZBN43 belonged to Bacillus,and shared 99.79% homologue with the known species Bacillus selenatarsenatis.However,because of the incomplete sequence,the confidence level is just 46,so other corroborations are still required for grouping HZBN43 into an exact species.