| 3种鸢尾组织培养直接成苗培养基筛选 |
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| 引用本文： | 王鹏,王文静,牛小花,王建.3种鸢尾组织培养直接成苗培养基筛选[J].福建林业科技,2009,36(3). |
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| 作者姓名： | 王鹏 王文静 牛小花 王建 |
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| 作者单位： | 1. 郑州牧业工程高等专科学校,河南郑州,450011
2. 河南省黄河迎宾馆,河南郑州,450011 |
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| 摘 要： | 在仿自然气候条件下,采用培养苗形态指标综合分析的方法,对3种鸢尾组织培养直接成苗的培养基进行筛选。结果表明:最适合黄娃娃和不朽白一次性成苗的培养基为MS+KT 0.5 mg.L-1+IBA 1 mg.L-1+-αNAA 0.6 mg.L-1+蔗糖30 g.L-1+琼脂粉8 g.L-1+活性碳0.3%,pH 6.7;最适合血石一次性成苗的培养基为MS+KT 0.5 mg.L-1+IBA 1mg.L-1+-αNAA 0.2 mg.L-1+蔗糖30 g.L-1+琼脂粉8 g.L-1+活性碳0.3%,pH 6.7。
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| 关 键 词： | 鸢尾 组织培养 直接成苗 培养基 |
Culture Medium Filtration for Culturing 3 Varieties of Iris germanica into Seedling by Tissue Directly |
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| WANG Peng,WANG Wen-jing,NIU Xiao-hua,WANG Jian.Culture Medium Filtration for Culturing 3 Varieties of Iris germanica into Seedling by Tissue Directly[J].Journal of Fujian Forestry Science and Technology,2009,36(3). |
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| Authors: | WANG Peng WANG Wen-jing NIU Xiao-hua WANG Jian |
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| Institution: | WANG Peng1,WANG Wen-jing1,NIU Xiao-hua2,WANG Jian2(1.Zhengzhou College of Animal Husbandry Engineering,Zhengzhou 450011,He'nan,China,2.Yingbin Hotel of The Yellow River of Henan,China) |
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| Abstract: | Under the culture condition for imitating nature climate,through the integrated analytic ways of culture seeding form target,Culture medium for culturing 3 varieties of Iris germanica into seedling by tissue directly was filtrated.The results showed:the best adaption culture medium to I.germanica 'Sundoll' and I.germanica `Immortality' plant in one step was MS+KT 0.5 mg·L-1+IBA 1 mg·L-1+α-NAA 0.6 mg·L-1+sucrose 30 g·L-1+agar 8 g·L-1+activated carbon 0.3%,pH 6.7;the best adaption culture medium to I.germanica 'Bloodstone' plant in one step:MS+KT 0.5 mg·L-1+IBA 1 mg·L-1+α-NAA 0.2 mg·L-1+sucrose 30 g·L-1+agar 8 g·L-1+activated carbon 0.3%,pH 6.7. |
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| Keywords: | Iris germanica tissue culture culturing into seedling directly culture medium |
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