| 玉米苞叶黄酮的提取 纯化及其抗氧化性能研究 |
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| 引用本文： | 芮怀瑾,刘郁,白云,燕传勇,张锋,周颖,聂锦涛.玉米苞叶黄酮的提取 纯化及其抗氧化性能研究[J].农产品加工．学刊,2021(3). |
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| 作者姓名： | 芮怀瑾 刘郁 白云 燕传勇 张锋 周颖 聂锦涛 |
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| 作者单位： | 徐州工业职业技术学院化学工程学院;南京农业大学 |
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| 基金项目： | 徐州工业职业技术学院院级科研项目(XGY2019A003);徐州工业职业技术学院产业研发项目(KJCCYYF2019092306);江苏省大学生创新创业训练计划项目(201913107016Y)。 |
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| 摘 要： | 为了拓宽玉米苞叶高附加值利用途径,通过单因素试验和正交试验确定了玉米苞叶黄酮的最佳提取条件,并以维E和二丁基羟基甲苯(BHT)为对照,分析了纯化过的玉米苞叶黄酮的抗氧化性能。结果表明,超声波辅助提取法提取玉米苞叶的最佳提取条件为超声功率500 W,提取时间40 min,提取温度70℃,提取剂乙醇体积分数60%。在此条件下玉米苞叶黄酮得率为1.083%。经过D101型大孔树脂纯化后,玉米苞叶黄酮的纯度提高了1.779倍,玉米苞叶黄酮的DPPH自由基清除作用和还原能力优于维E,与BHT相似。因此,玉米苞叶黄酮具有较好的抗氧化能力,可作为天然抗氧化剂推广应用。
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| 关 键 词： | 玉米苞叶 黄酮 超声波 提取 抗氧化 |
Study on Extraction and Purification of Flavonoids from Corn-bract and its Antioxidant Activity |
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| RUI Huaijin,LIU Yu,BAI Yun,YAN Chuanyong,ZHANG Feng,ZHOU Ying,NIE Jintao.Study on Extraction and Purification of Flavonoids from Corn-bract and its Antioxidant Activity[J].Nongchanpin Jlagong.Xuekan,2021(3). |
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| Authors: | RUI Huaijin LIU Yu BAI Yun YAN Chuanyong ZHANG Feng ZHOU Ying NIE Jintao |
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| Institution: | (College of Chemical Engineering,Xuzhou College of Industrial Technology,Xuzhou,Jiangsu 221140,China;Jiangsu Collaborative Innovation Center of Meat Production and Processing Quality and Safety Control,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China) |
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| Abstract: | The utilization approach of corn-bract with high additional value was widened.With singlefactor experiment and orthogonal experiment,the optimum extracting conditions of flavonoids from corn-bract were determined.VE and butylated hydroxy toluene(BHT)were used as study controls,while the antioxidant activity of purified flavonoids from corn-bract was analyzed.The results showed that the optimum conditions of ultrasonic-assisted extraction were 500 W of ultrasonic power,40 minutes of extraction time,70℃of extraction temperature,60%of ethanol concentration.Under these conditions,the yield of flavonoids was 1.083%.The purity of flavonoids from corn-bract was increased 1.779 times by macroporous resins D101.The DPPH free radical scavenging capability and reducing capacity of flavonoids were almost as effective as BHT,and better than VE.Therefore,flavonoids from corn-bract has good antioxidant capacity and can be used as natural antioxidants. |
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| Keywords: | corn-bract flavonoids ultrasonic extraction antioxidant |
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