水牛胚胎体外生产研究综述

近年来采用其它家畜上的程序来进行水牛体外胚胎生产（in vitro production，IVP）引起了越来越多的关注。水牛胚胎体外生产已获得了较高的卵母细胞成熟率、受精率和卵裂率，但囊胚发育率和体外胚的移植成功率仍较低，水牛IVP胚胎体外生产效率比黄牛低得多。在IVP技术应用于水牛生产育种之前，还有一些问题必须解决。本文对水牛胚胎体外生产的不同技术，包括未成熟卵母细胞的体外培养成熟和受精以及胚胎体外培养卵裂发育至囊胚等进行综述。文章还讨论了胚胎体外生产存在的问题及可能的解决方案。

in vitro production of buffalo embryos

Techniques for in vitro production (IVP) of buffalo embryos adopting the procedures developed in cattle have received increasing interest in the recent times. A high oocyte maturation, fertilization and cleavage rate and a low rate of blastocyst yield and calving following transfer of in vitro produced buffalo embryos have been obtained. The efficiency of IVP in buffalo is much lower than that in cattle. Several problems need to be resolved before IVP technology can be used regularly in buffalo breeding. This review attempts to present an overview of the different techniques used in buffalo to produce transferable embryos in vitro, namely in vitro maturation and fertilization of immature oocytes and in vitro development of the resulting cleaved embryos to the blastocyst stage before transfer. The problems associated with IVP and the possible solutions discussed.