| 用清除有机自由基DPPH法评价竹叶挥发油抗氧化能力 |
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| 引用本文： | 何跃君,岳永德,汤锋.用清除有机自由基DPPH法评价竹叶挥发油抗氧化能力[J].安徽农业大学学报,2009,36(3). |
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| 作者姓名： | 何跃君 岳永德 汤锋 |
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| 作者单位： | 国际竹藤网络中心,北京,100102 |
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| 基金项目： | “十一五”国家科技支撑计划项目子课题(2006BAD19B08);;国际竹藤网络中心基本科研业务费专项资金(06107-C27)共同资助 |
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| 摘 要： | 采用水蒸气蒸馏法从15种竹叶中提取挥发油,以TBHQ为对照,用DPPH·法研究竹叶挥发油对自由基的清除作用.结果表明,绿竹竹叶挥发油的得率最高为0.637%,而福建茶竿竹竹叶挥发油的得率仅为0.252%,水蒸气蒸馏法适宜于提取竹叶挥发油.15种竹叶挥发油均有一定的抗氧化活性,竹叶挥发油的抗氧化活性与挥发油的浓度呈正相关性.其中银丝竹竹叶挥发油的抗氧化活性最强,,IC50值为3.605 mg·mL-1,绿竹竹叶挥发油的IC50值为4.464 mg·mL-1,短穗竹竹叶挥发油抗氧化活性最低,其IC50值为12.128 mg·mL-1.综合研究结果表明,竹叶挥发油具有较高的应用价值,可作为天然抗氧化剂进一步开发和利用.
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| 关 键 词： | 竹叶 挥发油 1 1-二苯基苦基苯肼 抗氧化活性 |
Detection of antioxidative capacity of essential oils from the bamboo leaves by scavenging organic free radical DPPH |
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| HE Yue-jun,YUE Yong-de,TANG Feng.Detection of antioxidative capacity of essential oils from the bamboo leaves by scavenging organic free radical DPPH[J].Journal of Anhui Agricultural University,2009,36(3). |
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| Authors: | HE Yue-jun YUE Yong-de TANG Feng |
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| Institution: | International Center for Bamboo and Rattan;Beijing 100102 |
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| Abstract: | The antioxidant capacities of essential oils obtained by steam distillation from the leaves of the 15 bamboo species,were evaluated using the DPPH assays.The yield of oils from the leaves of the 15 bamboo species was variable with the greatest amount obtained from Dendrocalamopsis oldhami(0.637%),and the least from Pseudosasa amabilis(0.252%).In the DPPH assays,the antioxidant capacity was evident in all the oils,but the antioxidant capacity of essential oil that obtained from Bambusa multiplex(IC50=3.605 m... |
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| Keywords: | bamboo leaves essential oil DPPH antioxidant activity |
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