| 仔猪副伤寒活疫苗半合成培养基的配方筛选试验 |
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| 引用本文： | 马振宇,张大鹏,刘欣.仔猪副伤寒活疫苗半合成培养基的配方筛选试验[J].中国兽药杂志,2005,39(4):13-14. |
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| 作者姓名： | 马振宇 张大鹏 刘欣 |
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| 作者单位： | 1. 辽宁省益康生物制品厂,辽宁,辽阳,1110000
2. 辽宁省畜牧兽医科学研究所,辽宁,辽阳,111000 |
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| 摘 要： | 研制了一种适用于仔猪副伤寒活疫苗生产的半合成培养基，解决了培养基质量不稳定的问题。选用几种营养成分配比，制成半合成培养基进行细菌培养并冻干，结果冻前菌数超过普通肉汤培养基，冻干存活率略低于普通肉汤培养基，安全性检验合格，证明该培养基替代现用培养基生产疫苗是可行的。
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| 关 键 词： | 半合成培养基 仔猪副伤寒 配方筛选试验 活疫苗 安全性检验 疫苗生产 成分配比 细菌培养 不稳定 存活率 冻干 肉汤 营养 |
| 文章编号： | 1002-1280(2005)04-0013-02 |
| 修稿时间： | 2004年11月1日 |
Screening the semi-composed culture medium used in producing paratyphus living vaccine for piglets |
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| MA Zhen-yu,ZHANG Da-peng,LIU Xin.Screening the semi-composed culture medium used in producing paratyphus living vaccine for piglets[J].Chinese Journal of Veterinary Drug,2005,39(4):13-14. |
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| Authors: | MA Zhen-yu ZHANG Da-peng LIU Xin |
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| Abstract: | In order to dissolve the instability of the culture medium during the production of paratyphus living vaccine for piglets, we developed a kind of semi-composed culture medium. Selecting different proportion of several components, the semi-composed culture medium was used to culture bacteria, and the bacteria was then lyophilized. Before lyophilization, the number of the bacteria cultured with semi-compsoed cultured medium was higher than that of the conventional culture medium, and the livability was a little lower than that of the conventional (cultured) medium after lyophiliastion. The safety assay of the vaccine was eligible. So the semi-composed culture medium could replace the conventional culture medium. |
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| Keywords: | piglet paratyphoid living vaccine semi-composed culture medium |
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