Direct evidence of nitric oxide production induced by lactoferrin and its enhancement by magnesium ions in cultured endothelial cells |
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| Authors: | Tomoaki NII Md Zahorul ISLAM Satoru KAKE Mitsuya SHIRAISHI Takashi TAKEUCHI Hidefumi KUWATA Atsushi MIYAMOTO Etsumori HARADA |
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| Affiliation: | 1)Department of Veterinary Pharmacology, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan;2)Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh;3)Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan;4)NRL Pharma. Inc., Kanagawa, Japan;5)Tottori University, Tottori, Japan |
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| Abstract: | ![]()
Bovine lactoferrin (BLF) reportedly lowers blood pressure and induces vasorelaxation, but its effect on nitric oxide (NO) production has not been established. Accordingly, we aimed to determine whether BLF induces NO production in bovine aortic endothelial cells, and the effects of extracellular free magnesium (Mg) ion concentrations on this NO production. BLF induced NO production time-dependently. NO production was markedly inhibited by the NO synthase inhibitor, NG-nitro-L-arginine methyl ester, in an effect abolished by L-arginine, but not D-arginine. NO production was suppressed at low concentrations, and enhanced at high concentrations, of Mg ions in culture medium. These results suggest that BLF has an important role in hypotensive effects. Mg ions may affect BLF-induced NO production. |
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| Keywords: | bovine aorta endothelial cell cell culture lactoferrin magnesium nitric oxide production |
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