Critical diagnostic and cancer stem cell markers in neoplastic cells from canine primary and xenografted pulmonary adenocarcinoma |
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| Authors: | Warisraporn Tangchang Yun-Hyeok Kim Ye-In Oh Byung-Woo Lee Hyunwook Kim Byungil Yoon |
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| Affiliation: | 1.College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Korea.;2.Department of Veterinary Internal Medicine, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea.;3.Biotoxtech, Cheongju 28115, Korea.;4.Haemaru Animal Hospital, Seongnam 13590, Korea. |
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| Abstract: | ![]()
It is challenging to diagnose metastatic tumors whose cellular morphology is different from the primary. We characterized canine primary pulmonary adenocarcinoma (PAC) and its xenografted tumors by histological and immunohistochemical analyses for critical diagnostic and cancer stem cell (CSC) markers. To generate a tumor xenograft model, we subsequently transplanted the tissue pieces from the PAC into athymic nude mice. Immunohistochemical examination was performed for diagnostic (TTF-1, Napsin A, and SP-A) and CSC markers (CD44 and CD133). The use of CSC markers together with diagnostic markers can improve the detection and diagnosis of canine primary and metastatic adenocarcinomas. |
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| Keywords: | Canine pulmonary adenocarcinoma diagnostic markers immunohistochemistry stem cell markers tumor xenograft model |
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