牛磺胆酸对小鼠体内氧自由基的影响

为了研究牛磺胆酸(TCA)对小鼠体内氧自由基代谢的影响,采用黄嘌呤氧化酶法、二硫代二硝基苯甲酸法、紫外分光光度法、硫代巴比妥酸法和酶联免疫特异性测定法分别测定了给药后小鼠血浆、肝脏和脑组织中总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-PX)、过氧化氢酶(CAT)的活力、脂质过氧化产物丙二醛(MDA)的含量及血浆中一氧化氯合酶(NOS)的活力.结果显示,低剂量TCA能显著提高血浆、肝脏和脑组织中GSP-PX活力及肝脏和脑组织中CAT活力,显著降低血浆中GSH-PX活力和脑组织中GSH-PX活力.高剂量的TCA能显著提高血浆中GSH-PX、肝脏和脑组织中T-SOD、GSH-PX及肝脏中CAT活力.低、高剂量的TCA能显著降低血浆、肝脏和脑组织中MDA的含量,以及提高血浆中NOS活力.结果证明,TCA的抗氧自由基作用与其提高体内SOD、GSH-PX、CAT、NOS的活力和降低MDA含量有关.

Effects of TCA on Metabolism of Oxygen Derived Free Radicals in Mice

To study effects of TCA on oxygen derived free radicals in mice,the xanthine oxidase method,DTNB method,ultraviolet spectrotometry,barbital acid method and enzyme-linked immunospecific assay method were adopted to determine the activities of total superoxide dismutase(T-SOD),glutathioneperoxidase(GSH-Px),catalase(CAT),NOS,and the content of maleicdialdehyde(MDA).The results showed that,TCA can increase the activity of GSP-PX remarkably in plasma,liver and brain at low dose and the activity of CAT in liver and brain,But decrease the activity of GSH-PX significantly in plasma and brain.The high dose of TCA can increase the activity of GSP-PX in plasma,T-SOD and GSHPX in liver and brain and CAT in liver remarkably.At both low and high dose,TCA can decrease the content of MDA significantly in plasma,liver and brain,but increase the activity of NOS.The results demonstrated that: the function of antioxidation of TCA may be due to the increase of activity of SOD,GSH-PX,CAT,NOS and decrease of MDA.