| 应用Sos恢复系统筛选与番木瓜环斑病毒CI互作的寄主因子 |
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| 引用本文： | 潘雪莹,沈文涛,言普,高乐,庹德财,黎小瑛,周鹏.应用Sos恢复系统筛选与番木瓜环斑病毒CI互作的寄主因子[J].热带作物学报,2011,32(2):251-256. |
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| 作者姓名： | 潘雪莹 沈文涛 言普 高乐 庹德财 黎小瑛 周鹏 |
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| 作者单位： | 1. 中国热带农业科学院热带作物生物技术研究所,海南海口571101;农业部热带作物生物技术重点实验室,海南海口571101;海南大学农学院,海南海口570228
2. 中国热带农业科学院热带作物生物技术研究所,海南海口571101,农业部热带作物生物技术重点实验室,海南海口571101 |
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| 基金项目： | 中央级科研院所基本科研业务费项目(No.ITTBZD07-32); 热带园艺植物资源与遗传改良教育部重点实验室教育部重点实验室(海南大学)开放基金项目 |
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| 摘 要： | 应用Sos恢复系统(Sos recruitment system)筛选可能与番木瓜环斑病毒CI(Cylindrical inclusion protein,圆柱形内含体蛋白)相互作用的寄主蛋白因子,通过营养缺陷、温度敏感筛选、回转验证、序列测定和同源比对分析,获得1种候选的互作寄主蛋白,该蛋白与植物的叶绿体延伸因子Tu(Elongation factor Tu,EF-Tu)具有较高的相似性,并对其在番木瓜环斑病毒(Papaya Ringspot Virus,PRSV)致病与植物防御过程中的功能进行初步分析。
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| 关 键 词： | 番木瓜环斑病毒 CI Sos恢复系统 蛋白互作 |
Screening of Proteins Interacting with PRSV CI in Carica papaya |
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| PAN Xueying,SHEN Wentao,YAN Pu,GAO Le,TUO Decai,LI Xiaoying and ZHOU Peng.Screening of Proteins Interacting with PRSV CI in Carica papaya[J].Chinese Journal of Tropical Crops,2011,32(2):251-256. |
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| Authors: | PAN Xueying SHEN Wentao YAN Pu GAO Le TUO Decai LI Xiaoying and ZHOU Peng |
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| Institution: | PAN Xueying1,2,SHEN Wentao1,YAN Pu1,GAO Le1,TUO Decai1,LI Xiaoying1,ZHOU Peng1,2 1 Institute of Topical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Science/ Ministry of Agriculture Key Biotechnology Laboratory for Tropical Crops,Haikou,Hainan 571101,2 College of Agronomy,Hainan University,Hainan 570228,China |
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| Abstract: | The cylindrical inclusion(CI) protein of Papaya ringspot virus(PRSV)is the key protein which is invovlved in virus replication and cell-to-cell movement.Using PRSV CI as the bait,one protein from a papaya plants cDNA library was identified by a Sos-Recruitment system.Sequence alignment analysis revealed that this host protein was highly homology to Elongation factor Tu(EF-Tu)from different plant species.The possible roles of this host protein in PRSV infection and host defense were discussed,which provided ... |
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| Keywords: | Papaya ringspot virus CI Sos recruitment system Protein interaction |
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