| 香蕉枯萎病菌2个生理小种MAPK基因的克隆及序列分析 |
| |
| 引用本文： | 杨旸,董章勇,王振中. 香蕉枯萎病菌2个生理小种MAPK基因的克隆及序列分析[J]. 热带作物学报, 2011, 32(1): 94-98. DOI: 10.3969/j.issn.1000-2561.2011.01.020 |
| |
| 作者姓名： | 杨旸 董章勇 王振中 |
| |
| 作者单位： | 华南农业大学植物病理生理学研究室,广东广州,510642 |
| |
| 摘 要： | 为了解香蕉枯萎病菌(Fusarium oxysporum f.sp.cubense)1号生理小种(race 1)和4号生理小种(race4)丝裂原活化蛋白激酶(MAPK)基因focMK1,focMK4的序列差异,根据番茄枯萎病(Fusarium oxysporum f.sp.lycopersici)的MAPK基因相关序列设计引物,利用PCR和RT-PCR技术,分别克隆了香蕉枯萎病菌2个生理小种的基因序列和开放阅读框。结果表明,所获得的2个MAPK基因均含有3个内含子和4个外显子,2个基因序列只有一个碱基差异,编码氨基酸355个,氨基酸组成无差异;根据生物信息学软件预测编码蛋白没有信号肽,具有2个功能位点,分子量和pI分别为41ku和6.47。该基因编码的蛋白具有高度保守性,与大多数植物致病菌的同源性都在90%以上,不同病原镰刀菌间的同源性接近100%。
|
| 关 键 词： | 香蕉枯萎病菌 MAPK基因 序列分析 |
Cloning and Comparative Analysis of MAPK Gene from Fusarium oxyporum f. sp. cubense Race 1 and 4 |
| |
| YANG Yang,DONG Zhangyong and WANG Zhenzhong. Cloning and Comparative Analysis of MAPK Gene from Fusarium oxyporum f. sp. cubense Race 1 and 4[J]. Chinese Journal of Tropical Crops, 2011, 32(1): 94-98. DOI: 10.3969/j.issn.1000-2561.2011.01.020 |
| |
| Authors: | YANG Yang DONG Zhangyong WANG Zhenzhong |
| |
| Affiliation: | YANG Yang,DONG Zhangyong,WANG Zhenzhong Laboratory of Physiological Plant Pathology,South China Agricultural University,Guangzhou 510642,China |
| |
| Abstract: | Vascular wilt of bananas is one of the most serious banana disease worldwide.The MAP kinase is a signal transducer,plays an important role in pathogenesis mechanism.According to the homologous gene sequence documented in Genbank,a pair of primers were designed.Using PCR and RT-PCR,the gene and open reading frame(ORF) from two races of Fusarium oxysporum f.sp.cubense were amplified respectively.The gene included 3 introns and 4 exons.The ORF encoded 355-amino acid polypeptide with 41 ku of calculated molecul... |
| |
| Keywords: | MAPK Fusarium wilt Fusarium oxyporum f.sp.cubense Comparative analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《热带作物学报》浏览原始摘要信息 |
|
点击此处可从《热带作物学报》下载全文 |
|
