| 四倍体芦笋花药培养技术初步研究 |
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| 引用本文： | 张元国,李芳,包艳存,王林武,徐立功,杨晓东. 四倍体芦笋花药培养技术初步研究[J]. 山东农业科学, 2013, 45(2): 50-52 |
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| 作者姓名： | 张元国 李芳 包艳存 王林武 徐立功 杨晓东 |
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| 作者单位： | 潍坊市农业科学院,山东潍坊,261071 |
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| 基金项目： | 山东省现代农业产业技术体系蔬菜产业创新团队建设项目(2009-2012) |
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| 摘 要： | 以四倍体芦笋为试材,研究了花蕾不同长度及培养条件对芦笋花药培养的影响。结果表明:(1)四倍体芦笋花蕾长度为2.0～2.5 mm时,其诱导效果最好;(2)pH值为6.0时愈伤组织诱导率最高;(3)接种时间以4月底最好;(4)适合于四倍体花药愈伤组织诱导的激素配比是2,4-D 0.5 mg/L,NAA 0.5 mg/L,6-BA 0.1 mg/L;(5)愈伤组织器官分化的培养激素配比是6-BA 1.0 mg/L、NAA 0.5 mg/L。
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| 关 键 词： | 芦笋 四倍体 花药培养 愈伤组织 诱导 分化 |
Preliminary Study on Tetraploid Asparagus Anther Culture Technique |
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| Zhang YuanGuo , Li Fang , Bao YanCun , Wang LinWu , Xu LiGong , Yang XiaoDong. Preliminary Study on Tetraploid Asparagus Anther Culture Technique[J]. Shandong Agricultural Sciences, 2013, 45(2): 50-52 |
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| Authors: | Zhang YuanGuo Li Fang Bao YanCun Wang LinWu Xu LiGong Yang XiaoDong |
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| Affiliation: | (Weifang Academy of Agricultural Sciences,Weifang 261071,China) |
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| Abstract: | The tetraploid asparagus was used to study the effects of bud length and culture conditions on anther culture.The results were as follows.(1) When the tetraploid asparagus bud length was 2.0~2.5 mm,the induction effect was the best;(2) The callus induction rate was the highest,when the pH value was 6.0;(3) The end of April was the best inoculation time;(4) The suitable hormone proportion for callus induction was 2,4-D 0.5 mg/L,NAA 0.5 mg/L and 6-BA 0.1 mg/L;(5) The suitable hormone proportion for callus differentiation was 6-BA 1.0 mg/L and NAA 0.5 mg/L. |
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| Keywords: | Asparagus Tetraploid Anther culture Callus Induction Differentiation |
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