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| 麻疯树cpSSR标记技术的建立与体系优化研究 | |
| 引用本文： | 向倩,周兰英,万静,张旭,雷宝盛,金银春,冯毅,赵晓英,于绪任. 麻疯树cpSSR标记技术的建立与体系优化研究[J]. 安徽农业科学, 2009, 37(32): 15746-15748 |
| 作者姓名： | 向倩 周兰英 万静 张旭 雷宝盛 金银春 冯毅 赵晓英 于绪任 |
| 作者单位： | 四川农业大学林学院,四川雅安,625014 |
| 摘 要： | [目的]建立麻疯树cpSSR—PCR反应的最佳反应体系。[方法]对影响麻疯树cpSSR—PCR反应体系的5个因素（细DNA聚合酶、DNA模板、Mg2＋、dNTP、引物）进行优化试验，筛选各反应因素的最佳水平。[结果]20山反应体系各组分的最合适浓度分别为10XBuffer、2．00mmoL／LMg2＋、2U／μlTagDNA聚合酶、0．2mmol／LdNTP、0．2μmol／L引物、35ng／μlDNA模板。[结论]最佳的退火温度为52℃：该反应体系的稳定性和可重复性均较好。 |
| 关 键 词： | 麻疯树 cpSSR—PCR 体系优化 |
Study on Establishment of cpSSR Marker Technology and Optimization of Its Reaction System in Jatropha curcas Linn |
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| XIANG Qian et al. Study on Establishment of cpSSR Marker Technology and Optimization of Its Reaction System in Jatropha curcas Linn[J]. Journal of Anhui Agricultural Sciences, 2009, 37(32): 15746-15748 | |
| Authors: | XIANG Qian et al |
| Affiliation: | XIANG Qian et al (College of Forestry , Horticulture,Sichuan Agricultural University,Yaan,Sichuan 625014) |
| Abstract: | [Objective] The aim was to build an optimal cpSSR-PCR system for Jatropha curcas Linn. [Method] Optimize cpSSR-PCR amplification system for Jatropha curcas Linn in terms of five factors( Taq DNA polymerase, Mg~(2+), DNA template, dNTP and primer)from several levels. [Result] An optimal cpSSR-PCR system for Jatropha curcas Linn was obtained as 2.00 mmol/L Mg~(2+), 2 U/μl Taq DNApolymerase, 0.2 mmol/L dNTP, 0.2 μmol/L Primer, 35 ng/μl DNA template for 20 μl reaction syetem. [Conclusion] The optimal annealing temperature for cpSSR-PCR reaction syetem is determined as 52 ℃. The cpSSR reaction system was steady and reproducible. |
| Keywords: | cpSSR-PCR Jatropha curcas Linn cpSSR marker Optimization of reaction system |
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