| 植物染色体微分离微克隆技术研究进展与展望 |
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| 引用本文： | 吕柳新,黄代青.植物染色体微分离微克隆技术研究进展与展望[J].福建农林大学学报(自然科学版),2001,30(3):340-346. |
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| 作者姓名： | 吕柳新 黄代青 |
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| 作者单位： | 1. 福建农林大学园艺学院,
2. 福建农林大学园艺学院;福建师范大学生物工程学院 |
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| 摘 要： | 染色体微分离与微克隆技术是细胞遗传学与分子遗传学相结合的一项桥梁技术 ,近年来在植物中的应用日渐活跃 .本文综述了该技术在植物中的研究进展 ,并对其应用前景作了展望
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| 关 键 词： | 植物染色体 微切割微分离 微克隆 染色体文库 |
| 文章编号： | 1006-7817-(2001)03-0340-07 |
| 修稿时间： | 2001年6月27日 |
Advances and prospects in microdissection and microcloning of plant chromosomes |
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| LU Liu xin ,HUANG Dai qing.Advances and prospects in microdissection and microcloning of plant chromosomes[J].Journal of Fujian Agricultural and Forestry University,2001,30(3):340-346. |
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| Authors: | LU Liu xin HUANG Dai qing |
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| Institution: | LU Liu xin 1,HUANG Dai qing 1,2 |
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| Abstract: | The technique of chromosome microdissection and microcloning has been becoming an efficient tool for constructing chromosome or chromosome segment specific DNA libraries since the first successful cloning of specific chromosome regions in 1981. Now, it is widely used in the studies of genetics, medicine, evolution and other fields. In this paper, the development and research progress of this technique in plant are reviewed, including chromosome preparation, chromosome identification, microdissection, microcloning, confirmation of the clones and characterization of chromosome DNA libraries. The application prospects of this technique in plant are also discussed. |
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| Keywords: | plant chromosome microdissection microcloning chromosome DNA library |
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