| 叶锈菌诱导的小麦叶片cDNA文库的构建及其质量评价 |
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| 引用本文： | 陈云芳,杨文香,刘大群. 叶锈菌诱导的小麦叶片cDNA文库的构建及其质量评价[J]. 分子植物育种, 2009, 7(4): 699-702. DOI: 10.3969/mpb.007.000699 |
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| 作者姓名： | 陈云芳 杨文香 刘大群 |
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| 作者单位： | 1. 苏州出入境检验检疫局,苏州,215126;河北农业大学植物保护学院植物病理系,河北省农作物病虫害生物防治工程技术研究中心,保定,071001
2. 河北农业大学植物保护学院植物病理系,河北省农作物病虫害生物防治工程技术研究中心,保定,071001 |
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| 摘 要： | 由小麦叶锈菌(Puccinia triticina)引起的小麦叶锈病在世界各地产麦区均有发生,利用抗病品种是防治该病害最经济、安全、有效的方法.小麦抗叶锈基因Lr19是一个十分有效的抗叶锈基因,自1966年首次将该基因从长穗偃麦草转到普通小麦中,至今仍是一个应用潜力很大的抗病基因.本研究以小麦抗叶锈病近等基因系TcLr19和叶锈菌04-15-8①(生理小种类型THTS)为材料,构建叶锈病菌诱导的小麦叶片cDNA文库.研究结果表明文库的克隆数为4.1×106,插入片段大小在0.5～3.0kb,平均插入片段大小1.0 kb.因此该文库可用于基因克隆与分析,为研究小麦抗叶锈病相关基因提供条件.
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| 关 键 词： | 小麦抗叶锈基因Lr19 cDNA文库 构建和评估 |
Construction and Quality Identification of cDNA Library from Wheat Leaves Challenged by Puccinia recondita |
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| Chen Yunfang,Yang Wenxiang,Liu Daqun. Construction and Quality Identification of cDNA Library from Wheat Leaves Challenged by Puccinia recondita[J]. Molecular Plant Breeding, 2009, 7(4): 699-702. DOI: 10.3969/mpb.007.000699 |
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| Authors: | Chen Yunfang Yang Wenxiang Liu Daqun |
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| Affiliation: | Chen Yunfang 1,2 Yang Wenxiang 2 Liu Daqun 2 1 Suzhou Entry-Exit Inspection , Quarantine Bureau,Suzhou,215126,2 Department of Plant Pathology,College of Plant Protection,Hebei Agricultural University,Biological Control Center of Plant Diseases , Pests of Hebei Province,Baoding,071001 |
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| Abstract: | Wheat leaf rust caused by Puccinia triticina is a serious and widely distributed disease of wheat. Resistant cultivars are the most economic, safety and environmental friendly way for minimizing the losses caused by the disease. As an important leaf rust resistance gene, Lr19 was firstly transferred into the wheat genome from Thinopyrum sp. in the form of the substitution line Agrusin 1966. At present, Lr19 was a resistance gene with great potential to be used for wheat production. A cDNA library was cons... |
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| Keywords: | Puccinia triticina Lr19 gene cDNA library Construction and evaluation |
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