狂犬病鼠源野毒M株核蛋白基因的克隆及序列分析

从感染狂犬病的鼠脑中快速提取细胞总RNA，用RT—PCR方法得到编码核蛋白完整结构基因的cDNA，进一步将此基因克隆入pGEM—T中，进行核苷酸序列的测定，并推导出氨基酸序列，将这一序列与国内外已发表的9株狂犬病病毒的NP全基因进行比较分析。结果表明狂犬病鼠源野毒M株与上述9株的同源性在71．0％～99、8％之间，氨基酸同源性在77．6％～99．6％之间，M株与CVS株无论核苷酸序列还是氨基酸序列同源性都最高，分别为99．8％和99．6％，而与CTN珠的核苷酸序列同源性较低，与Mokola株的核苷酸序列以及氨基酸序列同源性都最低，分别为71．0％和77．6％。本研究为进行狂犬病病毒的分子流行病学调查和研制狂犬病基因工程苗提供了理论依据。

Cloning and sequencing of N gene of strain M of rabies virus isolated from mouse

The nucleoprotein structural gene of rabies virus M strain was amplified by RT-PCR from genomic RNA and cloned into pGEM-T plasmid vector and sequenced. Comparison and analysis of the nucleotide sequence and amino acid sequence with those of other 9 strains previously published were performed by computer with DNAStar sottware. The results showed that the nucleotide homology of them ranged from 71.0 % to 99.8 % and deduced amino acid sequence homology from 77.6 % to 99.6 %. Both cDNA sequence and the deduced amino acid sequence homology of strain M with CVS are the highest and with Mokola are the lowest. The nucleotide sequence homology of strain M with CTN is lower. The results play an important role for the investigation of molecular epidemiology and the study on looking for suitable genetic engineering vaccine against rabies virus.