乌拉尔甘草四倍体的离体诱导及倍性鉴定

为获得乌拉尔甘草四倍体新种质,本研究在离体培养条件下,利用不同浓度秋水仙素处理乌拉尔甘草(2n =2x=16)带腋芽茎段诱导四倍体,并进行了再生苗根尖染色体鉴定和气孔观察。结果表明:四倍体优化诱导体系为以0.1%秋水仙素处理24 h时诱导频率较高,变异率达到11%;再生植株气孔保卫细胞长度增长明显,气孔保卫细胞密度增长极其明显,气孔保卫细胞宽度有所下降,经根尖染色体鉴定,确定染色体为2n=4x=32,为同源四倍体植株。

The inducement and identification of autotetroploid of Glycyrrhiza uralensis Fisch cultured in vitro

In order to obtain the Glycyrrhiza uralensis Fisch tetraploid new idioplasm,this re- search,under the culture in vitro condition,the different process time condition,used different density colchicine solution to process the Glycyrrhiza uralensis Fisch(2n=2x=16)the axillury buds section induction tetraploid(2n=4x=32),and carried out the chromosome identification and the stomatal guardcells observation.The results indicated:with the colchicin density of 0.1%,pro- cess time 24 h,the inducing frequency was high,and the variation rate was 11%; the stomatal guardcells length growth was obvious,the stomatal density grew extremely obviously,and the sto- matal guardcells width dropped Considerably.