新疆棉花根际土壤铁载体产生菌的遗传多样性及系统发育研究

 【目的】了解中国新疆地区铁载体产生菌的遗传多样性和系统发育，为筛选高效铁载体产生菌提供依据。【方法】采用BOXAIR-PCR、16S rRNA PCR-RFLP和16S rDNA全序列分析方法对分离自新疆地区棉花根际土壤的铁载体产生菌的遗传多样性进行分析。【结果】在BOXAIR-PCR分析中，68株供试菌分为11个遗传群;在16S rRNA PCR-RFLP分析中，68株供试菌分10个遗传群;代表菌株的16S rDNA 全序列分析表明，供试菌分属于假单胞菌属（Pseudomonas）、肠杆菌属（Enterobacter）、不动细菌属（Acinetobacter）、芽孢杆菌属（Bacillus）、产碱杆菌属（Alcaligenes）、短状杆菌属（Brachybacterium）和泛菌属（Pantoea）。其中，假单胞菌属（Pseudomonas）为优势属。【结论】分离自新疆地区棉花根际土壤铁载体菌存在极大的遗传多样性。

Genetic Diversity and Phylogeny of Siderophore Producing Bacteria Isolated from Cotton Rhizosphere in China's Xinjiang

【Objecive】 The aim of this paper is to reveal the genetic diversity of siderophore producing bacteria in Xinjiang provincial cotton rhizospheric soils, and to screen some effective siderophore strains. 【Method】 The genetic diversity of 68 siderophore strains isolated from cotton rhizospheric soil was analyzed by BOXAIR-PCR and 16S rRNA PCR-RFLP. Furthermore, the phylogeny of the representative strains was determined by 16S rRNA sequences analysis. 【Result】 Analysis of BOXAIR-PCR showed that all the strains were divided into 11 BOXAIR-PCR groups. The results of 16S rRNA PCR-RFLP analysis suggested that the genetic diversity was also existed and 10 genotypes were formed. The 16S rDNA sequence of representative strains was analyzed and the phylogenic tree was constructed, and the results revealed that these siderophores belonged to seven different genera, ie. Pseudomonas, Enterobacter, Acinetobacter, Bacillus, Alcaligenes, Brachybacterium and Pantoea, and strains belonged to genus of Pseudomonas was the predominant one among them. 【Conclusion】 The genetic diversity of the siderophore strains isolated from cotton rhizospheric soils in Xinjiang province is high.