禽白血病病毒J亚群自然感染商品蛋鸡致瘤性新特征

2009年8月,山东省邹城市某海兰褐蛋鸡群,160日龄发病,死亡率为7％.患鸡经大体剖检、病理组织学、PCR和免疫组织化学等检测,确诊为禽白血病病毒J亚群(ALV-J)感染.病理组织学检测发现,病鸡单独患血管瘤,或髓细胞瘤和纤维肉瘤多发性出现,由ALV-J自然感染引起同一鸡体出现髓细胞瘤和纤维肉瘤尚属国内外首次报道.肝脏研磨接种DF-1细胞培养7d后传3代,细胞无病变,ELISA检测感染细胞上清ALV p27抗原阳性,进一步确诊此鸡群为ALV感染.对病变严重的鸡进行病毒分离及ALV-J gp85基因同源性比较显示与原型株HPRS-103的同源性最高,达94.1％.本研究丰富了ALV-J感染的临床诊断依据,并为ALV-J在我国蛋鸡群中多潜能致瘤机制的研究提供了科学基础.

New Features of Tumors Induced by Avian Leukosis Virus Subgroup J in Layer Flock

In August 2009,A Hy-line layer chicken flock was infected at Zoucheng in Shandong Province,and mortality was up to 7% at age of 160-day.Three of illness chickens were presented and diagnosed as avian leukosis virus subgroup J(ALV-J) infection by gross and histopathological observation,PCR and immunohistochemistry,etc.Histopathological observation showed that hemagioendotheliosarcoma existed single in No.1 chicken;fibrosarcoma was concurrent with myelocytomas in No.2 chicken.No cytopathic effect was found on the cultured DF-1 cells inoculated with sick bird liver samples and of which supernatants fluid was positive for ALV p27 antigen by ELISA test.Phylogenetic analysis showed that gp85 nucleotide sequences of the isolated strain had the highest homology with that of the prototype HPRS-103(94.1%).Our study demonstrated that ALV-J is a multipotential oncogenic virus.The mechanism of tumor spectrum expansion induced by ALV-J in layer flock needs to be further studied.