Iloprost对猪早期胚胎体外发育的影响

本研究旨在探讨PGI2类似物iloprost对猪胚胎体外发育的影响。试验以IVF胚胎为研究对象,分别在不同时期(0、24、48、72h)将不同浓度(0、0.2、0.5、1.0、2.0、5.0、10.0μmol.L-1)iloprost加入到猪胚胎培养液中,于156h时记录囊胚发育率和囊胚细胞数,筛选获得最佳添加方案,检测胚胎脂肪代谢速度和代谢相关基因(cox2、creb、pparδ、pdk、cpt2)的表达水平,分析iloprost影响胚胎发育的机制。结果显示,最佳的添加方案为,在受精后48h加入2.0μmol.L-1 iloprost,胚胎囊胚发育率(28%)和囊胚细胞数(49.42)显著高于(P<0.05)对照组的囊胚发育率(16%)和囊胚细胞数(28.22);添加iloprost后,胚胎脂肪酸降解速度也显著加快(P<0.05),脂肪酸代谢相关基因cox2、creb、pparδ、cpt2的表达量上升,糖代谢相关基因pdk表达量无显著变化。结果表明,PGI2类似物iloprost可以促进胚胎降解脂肪酸,为胚胎发育提供能量,提高了胚胎体外发育能力。

Effects of Iloprost on in vitro Development of Preimplantation Porcine Embryos

The aim of this study was to discuss the effect of PGI2 analogue iloprost on in vitro development of porcine embryos.In this study,at different times(0,24,48,72 h),iloprost with different concentrations(0,0.2,0.5,1.0,2.0,5.0,10.0 μmol·L-1)was added into the porcine zygote medium 3(PZM-3) to observe the effect of iloprost on porcine IVF embryos and to screen the best additive dosage for embryo culture,blastocyst number and blastocyst cell number were counted at 156 h after fertilization.The speed of embryo fat metabolism and related genes(cox2,creb,pparδ,pdk,cpt2) expression level were tested for understanding the mechanism of iloprost promoting embryonic development.The results showed that addition of 2.0 μmol·L-1 iloprost at 48 hours after fertilization could significantly(P<0.05) improve the blastocyst rate(28%vs16%,P<0.05) and blastocyst cell number(49.42 vs 28.22,P<0.05),significantly accelerate fatty acid degradation speed(P<0.05) and promote the expression of lipid metabolism related genes(cox2,creb,pparδ,cpt2).However there’s no significant change in glucose metabolism related genes(pdk) expression level.These results demonstrates that iloprost can enhance the in vitro developmental capacity of porcine embryo by promoting fatty acid degradation.