| GA1,3,4,7酶联免疫检测法（ELISA）的建立 |
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| 引用本文： | 黄少白 周燮. GA1,3,4,7酶联免疫检测法（ELISA）的建立[J]. 华北农学报, 1993, 8(4): 46-51 |
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| 作者姓名： | 黄少白 周燮 |
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| 作者单位： | 南京农业大学农学系 210014江苏省农科院遗传生理研究所(黄少白)，南京农业大学农学系 210014(周燮) |
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| 摘 要： | 用碳二亚胺法将赤霉素Ａ４与卵清蛋白连接，形成ＧＡ４－ＯＶＡ复合物用于包板，利用ＧＡ４－Ｍｅ与ＧＡ４－０ＶＡ对兔抗ＧＡ４－ＰＡｂｓ竞争结合反应，建立了ＧＡ１，３，４，７间接酶闻免疫检测法。其灵敏度，检测范围和最小检测样品量分别为４５ｐｇ，０．２－２００ｎｇ和５０－５００ｍｇ，样品的平均回收率为９７％，批内和批间的变异系数分别为４．２％和４．８％。
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| 关 键 词： | 赤霉素 酶联免疫检测 作物 |
The Development of GA_(1,3,4,7) ELISA |
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| Huang Shaobai Zhou Xie. The Development of GA_(1,3,4,7) ELISA[J]. Acta Agriculturae Boreali-Sinica, 1993, 8(4): 46-51 |
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| Authors: | Huang Shaobai Zhou Xie |
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| Abstract: | GA4-Ovalbumin (OVA) is synthesized via 1- ethyl-3-(3 dimethylaminopropyl-) -carbodiimide hydrochloride (EDC). An indirect enzyme-linked-immunosorbent-assay (ELISA) is developed for the quantitation of GA1, 3,4,7,utilizing GA4-OVA as a coating agent. Sensitivity, measuring range and minimum weight of fresh sample for the assay are 45pg, 0.2-200ng and 50-500mg, respectively. The recovery rate of the sample is 97%, and intra-and inter-assay coefficients of variation are 4.2% and 4.8%, respectively. |
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| Keywords: | GA1 3 4 7 ELISA |
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