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| 鸡传染性支气管炎病毒RT-PCR检测方法的建立 | |
| 引用本文： | 程晓霞,王劭,朱小丽,陈仕龙,林锋强,陈少莺.鸡传染性支气管炎病毒RT-PCR检测方法的建立[J].中国农学通报,2009,25(5):14-17. |
| 作者姓名： | 程晓霞 王劭 朱小丽 陈仕龙 林锋强 陈少莺 |
| 作者单位： | 1. 福建省农业科学院畜牧兽医研究所,福州,350013;福建省畜禽疫病防治工程技术研究中心,福州,350013 2. 福建省农业科学院畜牧兽医研究所,福州,350013 3. 福建省农业科学院畜牧兽医研究所,福州,350013;福建省农业科学院畜牧兽医研究所,福州,350013 |
| 基金项目： | 省财政专项-福建省农业科学院科技创新团队建设基金 |
| 摘 要： | 根据GenBank上发表的鸡传染性支气管炎病毒（IBV）基因序列，针对IBV M基因全序列设计合成了一对引物, 以IBV疫苗株为模板, 建立了检测IBV的RT-PCR方法。应用该方法对IBV疫苗株RNA进行扩增, 获得与预期大小相符, 长度为1105bp的特异性目的片段；敏感性测定该RT-PCR可扩增到18pg的IBV-M-cDNA。结果表明建立的RT-PCR方法对IBV的检测敏感性高、特异性强，可用于IBV感染的诊断和流行病学调查。 |
| 关 键 词： | 三峡库区 三峡库区 消落区 对策 |
| 收稿时间： | 2008-11-27 |
| 修稿时间： | 2009-01-12 |
Development of RT-PCR method for detecting Infectious Bronchitis Virus |
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| Cheng Xiaoxi,Wang Shao,Zhu Xiaoli,Cheng Shilong,Lin Fengqiang,Chen Shaoying.Development of RT-PCR method for detecting Infectious Bronchitis Virus[J].Chinese Agricultural Science Bulletin,2009,25(5):14-17. | |
| Authors: | Cheng Xiaoxi Wang Shao Zhu Xiaoli Cheng Shilong Lin Fengqiang Chen Shaoying |
| Institution: | Cheng Xiaoxia, Wang Shao, Zhu Xiaoli, Cheng Shilong, Lin Fengqiang, Chen Shaoying (1Institute of Animal Husbandry and Veterinary Medicine, FuJian Academy of Agriculture Sciences, Fuzhou 350013; 2 Fujian Animal Diseases Control Technology Development Center, Fuzhou 350013) |
| Abstract: | A pair of primers were designed and synthesized based on the sequences of the IBV genomes, and the RT-PCR method to detect the infectious Bronchitis virus （IBV）were developed.The 1105 bp specifical fragment were obtained from the IBV vaccine strain RNA by this RT-PCR.The sensitivity of RT-PCR reached to 2.85 ng IBV-M-cDNA.These results showed that the RT-PCR method to detect IBV were of better specificity and higher sensibility. The results showed that this PCR technique was specific and applied to IBV diagnoses and epidemiology investigation． |
| Keywords: | RT-PCR |
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