金樱子多糖对小鼠急性药物肝损伤的保护作用

为探讨金樱子多糖对CCl4所致小鼠急性肝损伤的保护作用。建立CCl4诱导的小鼠急性肝损伤模型，分光光度法检测丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、丙二醛(MDA)和还原型谷胱甘肽(GSH)含量，以及超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH2Px)的活性。结果表明：高剂量的金樱子多糖可显著降低CCl4所致急性肝损伤小鼠的肝脏指数(P<0.05)和脾脏指数(P<0.01)；中、高剂量的金樱子多糖可显著降低CCl4所致急性肝损伤小鼠血清ALT、AST、ALP活性(P<0.01)和肝组织MDA含量(P<0.01)，提高肝组织ALT、AST、ALP活性(P<0.01)和GSH-Px、SOD活性（P<0.01或P<0.05），并增加GSH的含量(P<0.01)。金樱子多糖对CCl4所致小鼠急性肝损伤具有一定的保护作用。

Protective Effect of Rosa laevigata Michx Polysaccharide on Acute Hepatic Injury Mice

To observe the hepatoprotective effects of Rosa laevigata Michx polysaccharide (RLP) on acute hepatic injury mice induced by carbon tetrachloride (CCl4). The acute hepatic injury mice of model was induced by CCl4. The levels of alkaline phosphatase (ALP), aspartate transminase (AST), alanine aminotransferase (ALT), malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were assayed by spectrophotometry in order to observe hepatoprotective effect of RLP. The results showed that, High-dosage RLP could significantly decreased liver index (P<0.05) and spleen index (P<0.01). Both high-dosage RLP and middle-dosage RLP could significantly decreased ALP, AST and ALT in the serum (P<0.01), but that of in liver tissue was just the opposite. And the above 2 groups could significantly decreased MDA and increased GSH-Px, GSH and SOD content (P<0.05 or P<0.01) in liver tissue of CCl4 poisoned mouse. RLP has hepatoprotective effect on acute hepatic injury in mice induced by CCl4.