利用染色体片段代换系定位陆地棉株高QTL

以陆地棉中棉所36为轮回亲本和海岛棉海1为供体亲本, 构建染色体片段代换系。为了能检测到稳定的株高QTL，将三个代换系群体(BC₅F₃, BC₅F_3:4和BC₅F_3:5)在5个环境中种植，2009年和2010年分别在河南安阳种植BC₅F₃单株、BC₅F_3:4株行， 2011年分别在河南安阳、辽宁辽阳和新疆石河子种植BC₅F_3:4株系。结果表明，在不同群体环境中株高的超亲比例为53.43%~88.97%。从早期构建的总图距为5088.28 cM， 含有2280个SSR标记位点，覆盖26条染色体的遗传连锁图谱中筛选标记，对408个单株进行的SSR鉴定，结果检测到16个株高QTL，分布在10条染色体上。单个QTL解释的表型变异为7.35%~13.17%。有7个QTL在2个以上环境被检测到。与标记MUSS563紧密连锁的qPH-15-19在一个环境中被检测到，在前人的研究中也有报道。这些结果为进一步精细定位QTL、基因克隆、分子辅助选择等研究奠定基础。

QTL Mapping for Plant Height Using Chromosome Segment Substitution Lines in Upland Cotton

Chromosome segment substitution lines (BC₅F₃, BC₅F_3:4, and BC₅F_3:5) were developed with G. hirsutum CCRI36 as the recipient parent and G. barbadense Hai1 as the donor parent. In this study, plant height of BC₅F₃ individuals (2009 in Anyang Henan), and BC₅F_3:4 lines (2010 in Anyang Henan) and BC₅F_3:5 lines (2011 in Anyang Henan, Shihezi Xinjiang and Liaoyang Liaoning, respectively) were evaluated. SSR markers were wed to screen 408 BC₅F₃ individuals. QTLs for plant height were identified by QTL IciMapping V3.2 software. The results indicated that the transgressive rate of plant height was from 53.43% to 88.97% and totally 16 QTLs for plant height were detected in three generations and five environments, which were mapped on 10 chromosomes, and explained the phenotypic variance from 7.35% to 13.17%. Seven QTLs were detected in at least two environments. qPH-15-19 was also reported in other researches. These stablly expressed QTLs could be applied in the marker assisted selection, fine QTL mapping and gene cloning.