| 花粉管通道法转化外源DNA的转基因技术 |
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| 引用本文： | 刘永巍,田红刚,李春光,程芳艳,孟昭河,杜金岭.花粉管通道法转化外源DNA的转基因技术[J].垦殖与稻作,2014(1):74-77,80. |
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| 作者姓名： | 刘永巍 田红刚 李春光 程芳艳 孟昭河 杜金岭 |
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| 作者单位： | 黑龙江省农垦科学院水稻研究所,黑龙江佳木斯154007 |
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| 摘 要： | 介绍了花粉管通道法的产生、分类及影响因素，阐述了花粉管通道法转基因技术的分子验证过程，列举了花粉管通道法在各作物育种上的应用，提出花粉管通道法应选择最佳导入方法与时期，从而提高外源DNA导人效率和遗传信息转化率，提高性状的变异频率，获得广泛的变异，提供丰富的变异材料。
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| 关 键 词： | 花粉管通道法 转基因技术 变异 应用 |
GM Technology of Exogenous DNA by Pollen-tube Pathway Method |
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| LIU Yong-wei,TIAN Hong-gang,LI Chun-guang,CHENG Fang-yan,MENG Zhao-he,DU Jin-ling.GM Technology of Exogenous DNA by Pollen-tube Pathway Method[J].Reclaiming and Rice Cultivation,2014(1):74-77,80. |
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| Authors: | LIU Yong-wei TIAN Hong-gang LI Chun-guang CHENG Fang-yan MENG Zhao-he DU Jin-ling |
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| Institution: | (Rice Research Institute,Heilongjiang Academy of Land Reclamation Sciences, Jiamusi Heilongjiang 154007,China) |
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| Abstract: | Generation process, classification and affecting factor of pollen-tube pathway method were introduced in paper, then it expounded the processes of molecular test and enumerated application to breeding of crops, it put forward that pollen-tube pathway method should select best import method and period, thus increase import efficiency and conversion of genetic information of exogenous DNA, increase mutant frequency of traits and obtain extensive mutation, offer rich mutant offspring. |
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| Keywords: | Pollen-tube pathway method GM technology Variation Application |
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